目的 選擇合適的中和劑消除增效復(fù)合胺消毒劑的抑菌作用，避免樣品中微生物限度檢查造成假陰性。方法 參照《中國藥典》2020年版四部微生物限度有關(guān)規(guī)定，聯(lián)合薄膜過濾法，分別采用3種中和劑配方去除季銨鹽類消毒劑抗菌活性。結(jié)果 采用組合配方中和劑（聚山梨酯、皂苷、卵磷脂、組氨酸比例30：30：3：1）并聯(lián)合薄膜過濾法可消除季銨鹽類消毒劑中的抑菌活性。結(jié)論 該方法所用中和劑組方經(jīng)濟(jì)易得，可為季銨鹽類消毒劑微生物限度檢查方法的建立提供依據(jù)。

Objective To select the appropriate neutralizing agent to eliminate the bacteriostatic effect of synergistic serotonin disinfectant and avoid false negative caused by microbial limit test in the sample. Methods Three neutralizer formulation were used to remove the antibacterial activity of quaternary ammonium salt disinfectant according to the applicability test of microbial limit count method provide by Chinese Pharmacopoeia (2020 Edition) volume Ⅳ and combined with the filtration and verification method. Results The method by using neutralizer formulation (Tween, saponin, lecithin, and histidine with proportion of 30:30:3:1) and filtration and verification method could eliminate the antibacterial activity of quaternary ammonium salt disinfectant. Conclusion The neutralizer formulation is economically and easily available, which can provide a basis for the establishment of microbial limit test method for quaternary ammonium salt disinfectants.

R943