目的 觀察舒腦欣滴丸對腦缺血模型大鼠恢復(fù)期的治療作用，并對其作用機制進(jìn)行探討。方法 采用線栓法制備大鼠局灶性腦缺血模型，給藥組ig給予舒腦欣滴丸（13、26、52 mg/kg），觀察其對模型大鼠恢復(fù)期各項指標(biāo)的影響。通過平衡木實驗考察大鼠肢體功能；通過前肢抓力測定，考察肌力功能；通過血清白細(xì)胞介素-1β（IL-1β）和IL-6測定，觀察炎癥因子變化；通過血小板激活因子（PAF）測定，觀察血小板活化狀態(tài)；通過腦組織HE染色，顯微鏡下觀察病理學(xué)改變，考察海馬、皮層神經(jīng)元數(shù)目以及病理學(xué)變化。結(jié)果 腦缺血損傷模型大鼠恢復(fù)期用舒腦欣滴丸治療后，受損的運動功能顯著改善，前肢抓力明顯增加，學(xué)習(xí)記憶能力明顯改善，血清內(nèi)炎癥因子IL-1β和IL-6水平顯著降低，海馬CA1區(qū)神經(jīng)元和皮層運動區(qū)神經(jīng)元均顯著增加。結(jié)論 舒腦欣滴丸ig給藥，對腦缺血模型大鼠恢復(fù)期有良好的治療作用，其治療作用機制可能與抑制炎癥因子以及減輕皮層運動區(qū)與海馬CA1區(qū)神經(jīng)元損傷有關(guān)。

Objective To observe the therapeutic effect of Shunaoxin Dropping Pill on the recovery period of cerebral ischemia model rats, and to explore its mechanism. Methods The model of focal cerebral ischemia in rats was induced by silk thread method, and Shunaoxin Dropping Pill was administrated by gavage to observe its effect on various indexes of recovery period. The limb function of rats was observed by balance beam test. The muscle strength function was measured through the measurement of the grasping force of the forelimb. IL-1 β and IL-6 were measured by ELISA to observe the changes of inflammatory factors. Platelet activating factor (PAF) was measured to observe platelet activation. The number of neurons and pathological changes in hippocampus and cortex were observed by HE staining. Results After the rats in the recovery period of cerebral ischemia injury were given Shunaoxin Dropping Pills, the motor function of rats was significantly improved, the grasping power of the forelimb and the learning and memory of the rats were significantly increased, the levels of IL-1 β and IL-6 in plasma decreased significantly, and the number of neurons in CA1 and motor cortex increased significantly. Conclusion Shunaoxin Dropping Pill has a good therapeutic effect on the recovery period of stroke, and its mechanism may be related with inhibition on inflammatory factors and reduction of injury of neurons in hippocampus and cortex.

R971

天津市第二批特支計劃青年拔尖人才基金項目（TJTZJH-QNBJRC-2-7）