目的 建立高效液相色譜測(cè)定人和小鼠糞便中短鏈脂肪酸的方法。方法 樣品進(jìn)行衍生化處理，采用高效液相色譜法測(cè)定乙酸、丙酸、丁酸以及戊酸。YMC-Pack FA型色譜柱（250 mm×6.0 mm，5 μm），流動(dòng)相甲醇-水（60：40，0.1%三氟乙酸調(diào)節(jié)pH值至4.5），檢測(cè)波長400 nm，體積流量1.0 mL/min，柱溫50℃，進(jìn)樣量20 μL。結(jié)果 乙酸、丙酸、丁酸、戊酸分別在0.300~6.005 mg/L（r=0.999 2）、0.370~7.408 mg/L（r=0.998 5）、0.441~8.811 mg/L（r=0.997 3）、0.511~10.210 mg/L（r=0.997 9）線性關(guān)系良好；平均加樣回收率分別為93.27%、96.40%、95.67%、95.43%，RSD值分別為4.62%、5.42%、3.64%、3.92%。結(jié)論 方法操作簡單，精密度、穩(wěn)定性、重復(fù)性良好，可用于測(cè)定人、小鼠糞便中短鏈脂肪酸。

Objective To establish an HPLC method for determination of short chain fatty acids in human and mice feces. Methods All samples were derived, and the contents of acetic acid, propionic acid, butyric acid, and pentanoic acid were determined by HPLC method. YMC-Pack FA chromatographic column (250 mm×6.0 mm, 5 μm) was used. The mobile phase consisted of methanol-water (0.2% trifluoroacetic acid solution regulated pH value to 4.5). The detection wavelengths were set at 400 nm. The flow rate was 1.0 mL/min, temperature of column was set at 50℃, and volume of injection was 20 μL. Results The linear range of acetic acid, propionic acid, butyric acid, and pentanoic acid were 0.300-6.005 mg/L (r=0.999 2), 0.370-7.408 mg/L (r=0.998 5), 0.441-8.811 mg/L (r=0.997 3), and 0.511-10.210 mg/L (r=0.997 9), respectively. The average recoveries were 93.27%, 96.40%, 95.67%, and 95.43%, respectively. The corresponding RSD values were 4.62%, 5.42%, 3.64%, and 3.92%, respectively. Conclusion The method is simple, accurate, stable, and reproducible, which can be used for the determination of short chain fatty acids in human and mice feces.

國家自然科學(xué)基金資助項(xiàng)目（81470796）