目的 通過挖掘Oncomine和GEO基因芯片數(shù)據(jù)庫(kù)中的相關(guān)數(shù)據(jù)，分析信號(hào)調(diào)節(jié)蛋白α（SIRPα）基因在乳腺癌中的表達(dá)及其臨床意義，并探討丹參酚酸B對(duì)乳腺癌細(xì)胞中SIRPα基因的干預(yù)作用。方法 檢索Oncomine和GEO數(shù)據(jù)庫(kù)中有關(guān)乳腺癌和丹參酚酸B干預(yù)乳腺癌的數(shù)據(jù)集，對(duì)所獲取的數(shù)據(jù)資料進(jìn)行數(shù)據(jù)挖掘，并結(jié)合文獻(xiàn)資料綜合分析。考察丹參酚酸B作用于乳腺癌MCF7細(xì)胞的基因表達(dá)譜。結(jié)果 對(duì)Oncomine數(shù)據(jù)庫(kù)中共收集的9項(xiàng)關(guān)于乳腺癌和乳腺正常組織SIRPα基因有差異表達(dá)的研究數(shù)據(jù)進(jìn)行分析，發(fā)現(xiàn)乳腺癌組織中SIRPα基因的表達(dá)明顯低于乳腺正常組織，差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。通過生存分析發(fā)現(xiàn)低表達(dá)SIRPα基因乳腺癌患者無病生存期明顯差于高表達(dá)者，高表達(dá)患者的預(yù)后更好（P<0.05）。通過挖掘GEO數(shù)據(jù)庫(kù)中的數(shù)據(jù)集GSE85871發(fā)現(xiàn)丹參酚酸B能夠上調(diào)乳腺癌細(xì)胞SIRPα基因的表達(dá)（P<0.01）。結(jié)論 SIRPα基因在乳腺癌組織中呈現(xiàn)低表達(dá)，并與乳腺癌患者的預(yù)后相關(guān)，該基因可能是丹參酚酸B干預(yù)乳腺癌細(xì)胞的一個(gè)新靶點(diǎn)。

Objective To analyze the expression and its clinical significance of signal regulatory protein α (SIRPα) gene in breast cancer through mining the related microarray data from Oncomine and GEO database, and to explore the effects of salvianolic acid B on the expression of SIRPα in breast cancer cells. Methods The datasets about breast cancer and effects of salvianolic acid B on breast cancer cell were retrieved from Oncomine and GEO database, and then the datasets were deeply mined and analyzed with literature. Gene expression profiles of salvianolic acid B against breast cancer MCF7 cells were investigated. Results The mRNA expressions of SIRPα gene in breast carcinoma tissues were significantly lower than those of breast normal tissues through mining and analyzing the 9 datasets about SIRPα gene in breast carcinoma from Oncomine database (P< 0.05). Survival analysis showed that the disease-free survival of breast cancer patients with higher expression of SIRPα gene was significantly worse than that of patients with lower expression of SIRPα, and the prognosis of patients with low expression of SIRPα gene was better (P < 0.05). The mRNA expression of SIRPα gene was down-regulated by salvianolic acid B in the datasets of GSE85871 through mining the GEO database (P< 0.01). Conclusion SIRPα gene is under-expressed in breast cancer and its expression level is related with the prognosis of breast cancer, which may be a new target of effects of salvianolic acid B on breast cancer cells.

國(guó)家自然科學(xué)基金資助項(xiàng)目（81704023、81602535）；湖北省衛(wèi)計(jì)委中西醫(yī)結(jié)合科研一般項(xiàng)目（23）；武漢市衛(wèi)計(jì)委科研項(xiàng)目（WZ15Z07）