目的 探討花仙宮血膠囊對功能失調(diào)性子宮出血大鼠模型子宮的保護(hù)作用。方法 采用米非司酮聯(lián)合米索前列醇建立大鼠功能失調(diào)性子宮出血模型。隨機(jī)分為模型組、宮血寧膠囊（0.13 g/kg）組以及花仙宮血膠囊0.23、0.46、0.92 g/kg組，每組各10只。對照組和模型組ig等體積蒸餾水，其余各組于受孕第5天開始給藥，每天給藥1次，體積均為10 mL/kg，連續(xù)給藥10 d。于末次給藥后，次日處死大鼠，迅速摘取子宮、卵巢，計(jì)算臟器系數(shù)。采用固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)進(jìn)行檢測內(nèi)膜血管內(nèi)皮生長因子（VEGF）、基質(zhì)金屬蛋白酶-1（MMP-1）表達(dá)水平；觀察HE染色后子宮組織形態(tài)學(xué)的變化。結(jié)果 花仙宮血膠囊0.23、0.46、0.92 g/kg對子宮和卵巢指數(shù)均有增加趨勢，但無顯著性差異?；ㄏ蓪m血膠囊0.46、0.92 g/kg明顯升高子宮組織的VEGF表達(dá)水平（P<0.01），0.23、0.46、0.92 g/kg明顯降低子宮組織的MMP-1表達(dá)水平（P<0.01）?；ㄏ蓪m血膠囊對間質(zhì)纖維結(jié)締組織增生和炎細(xì)胞浸潤有所改善，甚至可見新生血管。結(jié)論 花仙宮血膠囊對功能失調(diào)性子宮出血大鼠具有顯著的子宮保護(hù)作用，其作用機(jī)制與促進(jìn)性器官的生長和成熟，調(diào)節(jié)子宮內(nèi)膜VEGF、MMP-1表達(dá)水平有關(guān)。

Objective To discuss the protective effect of Huaxian Gongxue Capsules on uterus in dysfunctional uterine bleeding rats. Methods Dysfunctional uterine bleeding rat models were induced by misoprostol and mifepristone. Rats were randomly divided into model group, Gongxuening Capsules (0.13 g/kg) group, and Huaxian Gongxue Capsules (0.23, 0.46, and 0.92 g/kg) groups, and each group had 10 rats. Rats in the control and model group were given the same volume of water. The rest groups were ig administrated with the volume of 10 mL/kg from 5th day of pregnancy, first daily, and were treated for 10 d. After the last administration, rats were killed on the next day, and the uterus and ovaries were quickly removed, and the organ coefficient was calculated. The expressions of VEGF and MMP-1 in endometrium were detected by solid phase sandwich ELISA. Morphologic changes of uterus were observed after HE staining. Results Huaxian Gongxue Capsules could increase the index of uterus and ovary, but there was no significant difference. Huaxian Gongxue Capsules (0.46 and 0.92 g/kg) could significantly increase the levels of VEGF (P < 0.01) and Huaxian Gongxue Capsules (0.23, 0.46, and 0.92 g/kg) could decrease the levels of MMP-1 (P < 0.01). The proliferation of connective tissue and inflammatory cell infiltration in the interstitial fiber in Huaxian Gongxue Capsules groups were improved, and even the new blood vessels were visible. Conclusion Huaxian Gongxue Capsules have remarkable protection on uterus in dysfunctional uterine bleeding rats, and the mechanism is related to growth and maturation of sexual organs and the regulation of expression of VEGF and MMP-1 in the endometrium.

陜西省自然科學(xué)基礎(chǔ)研究計(jì)劃項(xiàng)目（2012JM4016）