目的 探討右歸飲對去勢雌性小鼠骨質(zhì)疏松的影響，并對其作用機(jī)制進(jìn)行研究。方法 將C57BL/6雌性小鼠去卵巢手術(shù)模擬骨質(zhì)疏松癥，飼養(yǎng)12周后，分別分為假手術(shù)組、模型組、雷洛昔芬組、右歸飲組，每組各9只，模型組、假手術(shù)組ig生理鹽水，右歸飲組ig右歸飲溶液。雷洛昔芬組ig鹽酸雷洛昔芬溶液10 mg/kg。每天每只均給藥0.2 mL，連續(xù)給藥5周，眼球取血，測定血清中生化指標(biāo)和鈣、磷水平；將動物處死，測定骨形態(tài)計(jì)量指標(biāo)和做Micro-CT。分離并培養(yǎng)骨內(nèi)間充質(zhì)干細(xì)胞，測定細(xì)胞的增殖和分化。結(jié)果 與模型組比較，右歸飲組的骨密度、骨體積分?jǐn)?shù)、骨小梁平均厚度、骨小梁數(shù)量均顯著升高，骨表面積/骨體積和骨小梁分離度均顯著降低，差異具有統(tǒng)計(jì)學(xué)意義（P<0.05）。右歸飲組I型膠原氨基端前肽（P1NP）水平較模型組顯著升高（P<0.05），膠原羧基端（CTx）水平比模型組略高一點(diǎn)，差異均無統(tǒng)計(jì)學(xué)意義。右歸飲組鈣、磷水平與模型組各指標(biāo)之間的差異均無統(tǒng)計(jì)學(xué)意義。與模型組比較，右歸飲組細(xì)胞呈增長趨勢，但增長率較其他組相對較低（P<0.01），倍數(shù)變化顯著升高（P<0.01）。結(jié)論 右歸飲對去勢雌性小鼠骨質(zhì)疏松有治療作用，可能是通過促進(jìn)間充質(zhì)干細(xì)胞分化成成骨細(xì)胞，從而刺激骨形成，達(dá)到有效抑制小鼠骨的丟失。

Objective To explore the therapeutic effect of Yougui Drink on osteoporosis in ovariectomized female mice and study its mechanisms. Methods The osteoporosis was mimicked by excising the ovary of C57BL/6 mice. After 12 weeks, random allocation was taken to averagely divide into Sham, model, raloxifene, and Yougui Drink e groups, and each group had 9 mice. Rats in the control group and model group were given the same volume of normal saline. Rats in Yougui Drink group were given Yougui Drink. Rats in raloxifene group were ig administered with Raloxifene Hydrochloride 10 mg/kg. All animals were given the volume 0.2 mL, 1 time/d, and treated for 5 weeks. The blood samples were taken from the eyeball, and serum biochemical indexes and levels of calcium and phosphorus were assessed. Mice were killed, and bone histomorphometry were assessed by Micro-CT. Bone mesenchymal stem cells were isolated and cultured and cell proliferation and ability of osteogenic differentiation were assessed. Results Compared with model group, systemic manifestations score, bone mineral density, bone volume fraction, the mean trabecular thickness, trabecular number in the Yougui Drink group were significantly increased, but bone surface area/bone volume and trabecular separation were significantly decreased (P<0.05). The levels of P1NP in the Yougui Drink group were significantly increased (P<0.05), but the CTx levels were slightly higher than those in the model group, the differences were not statistically significant. There were no significant differences on levels of calcium and phosphorus between the Yougui Drink group and the model group. Compared with the model group, the cells in the Yougui Drink group were increased, but the growth rates were lower than those in the other groups (P<0.01), and the changes of multiples were significantly higher (P<0.01). Conclusion Yougui Drink can treat osteoporosis in ovariectomized female mice, which may promote the differentiation of mesenchymal stem cells into osteoblasts, thereby stimulate bone formation, and effectively inhibit bone loss in mice.

國家自然科學(xué)基金資助項(xiàng)目（81460734）