目的 建立參芪消渴顆粒中尿囊素、24-乙酰澤瀉醇A、23-乙酰澤瀉醇B、茯苓酸和毛蕊異黃酮葡萄糖苷同時測定的方法。方法 采用高效液相色譜法，使用依利特Hypersil ODS 2色譜柱（250 mm×4.6 mm，5 μm）；流動相：乙腈–0.1%磷酸溶液，梯度洗脫；測定波長：0～14 min在224 nm波長下尿囊，14～20 min在208 nm波長下檢測24-乙酰澤瀉醇A、23-乙酰澤瀉醇B，20～26 min在210 nm波長下檢測茯苓酸，26～36 min在260 nm波長下檢測毛蕊異黃酮葡萄糖苷；體積流量：0.9 mL/min；柱溫：30 ℃；進(jìn)樣量：10 μL。結(jié)果 5個成分的線性范圍分別為9.43～188.60 μg/mL（r=0.999 4），3.75～75.00 μg/mL（r=0.999 7），4.06～81.20 μg/mL（r =0.999 9），4.82～96.40 μg/mL（r=0.999 5），3.79～75.80 μg/mL（r=0.999 8）。平均回收率分別為97.99%、99.34%、98.20%、96.57%、98.98%，RSD值分別為0.62%、1.14%、1.28%、0.79%、0.93%。結(jié)論 所建方法簡便、可靠、準(zhǔn)確度高，可有效地控制參芪消渴顆粒的質(zhì)量。

Objective To develop a method for simultaneous determination of allantoin, 24-acetate alisol A, 23-acetate alisol B, pachymic acid, and calycosin 7-O-β-D-glucopyranoside in Shenqi Xiaoke Granules. Method An HPLC method was adopted for the analysis on Hypersil ODS 2 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitril-0.1% phosphoric acid solution with gradient elution. The detection wavelengths were set at 224 nm in 0-14 min (determination of allantoin), 208 nm in 14-20 min (determination of 24-acetate alisol A and 23-acetate alisol B), 210 nm in 20-26 min (determination of pachymic acid), and 260 nm in 26-36 min (determination of calycosin 7-O-β-D-glucopyranoside). The flow rate was 0.9 mL/min, temperature of column was set at 30 ℃, and volume of injection was 10 μL. Results The linear range of allantoin, 24-acetate alisol A, 23-acetate alisol B, pachymic acid, and calycosin7-O-β-D-glucopyranoside were 9.43-188.60 μg/mL (r=0.999 4), 3.75-75.00 μg/mL (r=0.999 7), 4.06-81.20 μg/mL (r=0.999 9), 4.82-96.40 μg/mL (r=0.999 5), and 3.79-75.80 μg/mL (r=0.999 8), respectively. The average recoveries were 97.99%, 99.34%, 98.20%, 96.57%, and 98.98% with RSD 0.62%, 1.14%, 1.28%, 0.79%, and 0.93%, respectively. Conclusion The method is simple, reliable, and accurate, and can effectively control the quality of Shenqi Xiaoke Granules.