目的 評(píng)價(jià)澤瀉湯95%乙醇、50%乙醇和水提取物抑制HepG2肝細(xì)胞脂肪累積能力。方法 采用油酸誘導(dǎo)HepG2細(xì)胞脂肪累積建立模型，以噻唑蘭染色和油紅O染色法吸光度值為指標(biāo)，結(jié)合細(xì)胞內(nèi)脂滴形態(tài)，評(píng)價(jià)澤瀉湯對(duì)油酸誘導(dǎo)HepG2細(xì)胞脂肪累積的干預(yù)作用。結(jié)果 澤瀉湯提取物作用HepG2細(xì)胞后，經(jīng)染色后均發(fā)現(xiàn)細(xì)胞內(nèi)脂滴明顯減少，且質(zhì)量濃度越高脂類含量越少，50%乙醇提取物和水提物在終質(zhì)量濃度160、320 μg/mL時(shí)，細(xì)胞內(nèi)脂滴與模型組相比明顯降低（P<0.01），95%乙醇提物80 μg/mL作用細(xì)胞24 h后，細(xì)胞內(nèi)脂滴較模型組有減少（P<0.05）。結(jié)論 3種澤瀉湯提取物均能夠減少細(xì)胞內(nèi)脂肪的堆積，其中以50%乙醇提取物對(duì)HepG2細(xì)胞脂肪堆積抑制能力最強(qiáng)。

Objective To evaluate the effects of 95% ethanol, 50% ethanol, and distilled water extracts from Zexie Decoction on fat accumulation of HepG2 hepatic cells. Methods Fat accumulation model in HepG2 cells was induced by oleic acid. Absorptions of MTT method and oil red O method and intracellular lipid droplet morphology were used to evaluate the effect of Zexie Decoction on oleic acid-induced fat accumulation in HepG2 cells. Results The lipid droplet of HepG2 cells was significantly reduced after cultivation with Zexie Decoction extracts, and the higher concentration, the less lipid contents. After culturing with 50% ethanol extract (160 μg/mL) and water extract (320 μg/mL), the lipid droplet of HepG2 cells reduced significantly compared with model group (P<0.01), and after culturing with 95% ethanol extract with the maximum concentration of 80 μg/mL for 24 h. the lipid droplet of HepG2 cells was also reduced (P<0.05). Conclusion Three extracts of Zexie Decoction show the effect of lipid-decreasing, and 50% ethanol extract is the best lipid-lowering ingredient.

天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計(jì)劃項(xiàng)目（14JCQNJC13800；天津市科技計(jì)劃項(xiàng)目（14ZCZDSY00005）