目的 探究鹽知母不同溶劑萃取物對2型糖尿病大鼠糖代謝的影響,旨在尋找鹽知母降血糖作用的有效部位。方法 SD雄性大鼠ig脂肪乳10 g/kg,1次/d。4周后,ip鏈脲佐菌素35 mg/kg,建立2型糖尿病模型。造模成功的大鼠按血糖值隨機分為模型組、阿卡波糖(0.2 g/kg)組以及石油醚萃取物、氯仿萃取物、醋酸乙酯萃取物、正丁醇萃取和萃余物組,連續(xù)ig給藥14 d。于給藥7、14 d后測定空腹血糖值(FBG),末次給藥后測定口服葡萄糖耐量(OGTT),眼眶取血測定糖化血紅蛋白(HbAlc)、血清胰島素(FINS),計算胰島敏感指數(shù)(ISI)和Homa胰島素抵抗(HOMA-IR)指數(shù)。結果 與模型組比較,鹽知母氯仿萃取物能夠明顯降低FBG、HbA1c、FINS、HOMA-IR指數(shù),升高ISI,改善OGTT;鹽知母醋酸乙酯萃取物能夠顯著降低HbAlc、HOMA-IR指數(shù),改善OGTT;鹽知母正丁醇萃取物能夠顯著降低FINS;鹽知母萃余物能夠顯著降低FBG、FINS、HOMA-IR指數(shù),升高ISI。結論 鹽知母不同溶劑萃取物對2糖尿病大鼠糖代謝具有較好的改善作用,其中氯仿萃取物的作用最強。

Objective To explore the influence of various extracts from salt Anemarrhenae Rhizoma on glucose metabolism in rats with type 2 diabetes mellitus, and to find the effective part on the hypoglycemic effect. Methods Male SD rats were ig administered with fat milk 10 g/kg, once daily for 4 weeks, and established 2 type diabetic rat model by ip streptozotocin 35 mg/kg. Rats with type 2 diabetes mellitus were randomly divided into model group, acarbose (0.2 g/kg) group, and salt Anemarrhenae Rhizoma extracts groups of petroleum ether, chloroform, ethyl acetate, n-butanol, and raffinate. All animals were ig administered once daily for 14 d. Fasting blood glucose (FBG) was determined after treated for 7 and 14 d. After the last administration, the level of oral glucose tolerance test (OGTT) was determined. The blood samples were collected to measure the glycosylated hemoglobin (HbAlc) and fastings erum insulin (FINS), and insulin sensitivity index (ISI) and Homa insulin resistance (HOMA-IR) index were calculated. Results Compared with model group, chloroform extract group could significantly reduce the FBG, HbAlc, FINS, and HOMA IR index, increase ISI index and improve OGTT. Ethyl acetate extract could significantly reduce the HbAlc and HOMA IR index, and improve OGTT. n-Butanol extract could significantly reduce the content of FINS. Raffinate could significantly reduce the FBG, FINS, and HOMA IR index, and increase the ISI. Conclusion Various solvents extracts of salt Anemarrhenae Rhizoma can better improve glucose metabolism in rats with type 2 diabetes mellitus, and chloroform extract has stronger efficacy.

國家自然科學基金青年基金資助項目(81102810);遼寧省自然科學基金資助項目(2013020165)