目的 建立HPLC-ELSD法同時測定白果藥材中銀杏內(nèi)酯A、銀杏內(nèi)酯B和銀杏內(nèi)酯C 3個萜杏內(nèi)酯成分。方法 采用Agilent 5TC C₁₈色譜柱(250 mm×4.6 mm,5 μm),以甲醇-四氫呋喃-水(25:10:65)為流動相,體積流量1 mL/min,柱溫30 ℃,蒸發(fā)光散射檢測器檢測,對照品進樣量為10、20 μL,供試品溶液進樣量為5~20 μL。結(jié)果 銀杏內(nèi)酯A在0.37~5.92 μg、銀杏內(nèi)酯B在2.8~44.8 μg、銀杏內(nèi)酯C在0.65~10.4 μg時線性關系良好。銀杏內(nèi)酯A、銀杏內(nèi)酯B、銀杏內(nèi)酯C平均回收率分別為92.40%、95.03%、91.29%,RSD值分別為2.75%、2.06%、2.88%。結(jié)論 本法操作簡單,重復性好,為白果藥材的質(zhì)量控制提供了依據(jù)。

Objective To establish an HPLC-ELSD method for the determination of three terpene lactones ginkgolide A, ginkgolide B, and ginkgolide C in Ginkgo Semen. Methods The determination was carried out on Agilent 5TC C₁₈ column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-tetrahydrofuran-water (25:10:65). The column temperature was set at 30 ℃ at a flow rate of 1.0 mL/min. ELSD was used as detector to determine the terpene lactones. The injection volumes were 10 and 20 μL for reference substance solution, and 5—20 μL for sample solution. Results The linear ranges of ginkgolide A, ginkgolide B, and ginkgolide C were 0.37—5.92 μg, 2.8—44.8 μg, and 0.65—10.4 μg, respectively. The average recoveries of three components were 92.40%, 95.03%, and 91.29% with RSD 2.75%, 2.06%, and 2.88%, respectively. Conclusion The method is simple and repeatable, which can be used in quantity control for Ginkgo Semen.

國家重大新藥創(chuàng)制科技重大專項(2013ZX09402203)