目的 建立HPLC法同時(shí)測(cè)定三才封髓丸中地黃苷A、地黃苷D、黃柏堿和木蘭花堿的方法。方法 采用Phenomenex RP C₁₈色譜柱(250 mm×4.6 mm,5 μm);流動(dòng)相為乙腈-0.1%磷酸溶液,梯度洗脫;0～28 min地黃苷A和地黃苷D檢測(cè)波長(zhǎng)為203 nm,28～60 min黃柏堿和木蘭花堿的檢測(cè)波長(zhǎng)為225 nm;體積流量為1.0 mL/min;柱溫為30 ℃;進(jìn)樣量20 μL。結(jié)果 地黃苷A、地黃苷D、黃柏堿和木蘭花堿在4.52～90.40、4.10～82.00、5.35～107.00、5.28～105.60 μg/mL線性關(guān)系良好;平均回收率分別為98.86%、96.95%、99.15%、96.98%,RSD值分別為1.38%、1.24%、1.28%、0.85%。結(jié)論 建立的方法簡(jiǎn)便、準(zhǔn)確、靈敏度高、重復(fù)性好,可用于三才封髓丸的質(zhì)量控制。

Objective To establish an HPLC method for simultaneous determination of rehmannioside A, rehmannioside D, phellodendrine, and magnoflorine in Sancai Fengsui Pills. Methods The HPLC method was carried out on a Phenomenex RP C₁₈ column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisted of acetonitrile-0.1% phosphoric acid solution for gradient elution. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ with injection volume of 20 μL. The detection wavelength for rehmannioside A and rehmannioside D was set at 203 nm, and that for phellodendrine and magnoflorine was set at 225 nm. Results Rehmannioside A, rehmannioside D, phellodendrine, and magnoflorine had good linearity in the ranges of 4.52-90.40 μg/mL, 4.10-82.00 μg/mL, 5.35-107.00 μg/mL, and 5.28-105.60 μg/mL, respectively. And the average recoveries were 98.86%, 96.95%, 99.15%, and 96.98% with RSD values of 1.38%, 1.24%, 1.28%, and 0.85%, respectively. Conclusion The method is simple, accurate, and sensitive, with good repeatability, which can be used to evaluate the quality of Sancai Fengsui Pills.