目的 建立三七總皂苷中三七皂苷K的超高效液相色譜(UPLC)測(cè)定的方法。方法 采用Cosmosil Cholester色譜柱(50 mm×2.0 mm,2.5 μm);流動(dòng)相:[水–乙腈(85:15)]–乙腈,進(jìn)行梯度洗脫;體積流量0.5 mL/min;檢測(cè)波長(zhǎng)203 nm;柱溫30 ℃;進(jìn)樣量2 μL。結(jié)果 三七皂苷K在2.020～101.000 μg/mL與峰面積呈良好的線性關(guān)系(r=0.999 9),平均回收率為99.50%,RSD值為1.35%。結(jié)論 建立的UPLC法操作簡(jiǎn)便、結(jié)果可靠、重復(fù)性好、專屬性強(qiáng),可用于三七總皂苷中三七皂苷K的質(zhì)量控制。

Objective To establish a method for determination of notoginsenoside K in Notoginseng total saponins by ultra performance liquid chromatography (UPLC) method. Methods The determination was carried out on Cosmosil Cholester column (50 mm×2.0 mm, 2.5 μm). The mobile phase consisted of [water-acetonitrile (85:15)]-acetonitrile with gradient elution at a flow rate of 0.5 mL/min. The detection wavelength was set 203 nm and the column temperature was at 30 ℃ with injection volume of 2 μL. Results Notoginsenoside K had good linearity in the ranges of 2.020-101.000 μg/mL (r=0.999 9). The average recovery was 99.50% with RSD of 1.35%. Conclusion The method has simple operation, accurate results, good reproducibility, and specificity which can be used in the quantity control of notoginsenoside K in Notoginseng total saponins effectively.

云南省科技計(jì)劃項(xiàng)目(2012ZA002)