目的 考察黃花草木犀中蘆丁、clovin 和香豆素的最佳大孔吸附樹脂的純化工藝.方法 通過對(duì)不同型號(hào)大孔樹脂的考察優(yōu)選出純化黃花草木犀中藥效成分的最佳樹脂類型,并對(duì)其最大上樣量、上樣體積流量、洗脫溶劑體積分?jǐn)?shù)和洗脫溶劑最佳用量進(jìn)行優(yōu)化.結(jié)果 經(jīng)過篩選確定HPD-300 大孔樹脂為純化黃花草木犀藥效成分的最優(yōu)樹脂類型,其最佳純化條件:樹脂的上樣量為2.4 g 生藥/g 樹脂,上樣體積流量為2.0 BV/h,依次用2 BV 水洗脫和7 BV 50%乙醇洗脫.在此條件下蘆丁、clovin、香豆素質(zhì)量分?jǐn)?shù)提升了7.8 倍,平均收率為85.38%.結(jié)論 HPD-300 大孔樹脂用于純化黃花草木犀中蘆丁、clovin、香豆素穩(wěn)定性高、重復(fù)性好.

Objective To investigate the optimum purification process of the active ingredients rutin, clovin, and coumarin in Melilotus officinalis by macroporous resins. Methods The various types of resins were selected with purification of the active ingredients rutin, clovin, and coumarin in M. officinalis as index. The parameters, such as sample loading maximum volume, sample loading velocity, elution solvent concentration, and elution solvent volume of optimized resin were studied. Results HPD-300 type macroporous resin was selected as the best type of resin for the purification of active ingredients in M. officinalis. The optimum purification conditions were as following: the sample loading maximum volume was 2.4 g/g macroporous resin, sample loading velocity was 2.0 BV/h, and the sample was eluted by water with 2 BV and 50% ethanol with 7 BV, respectively. Rutin, clovin and coumarin proportion promoted 7.8 times after the purification HPD-300 by macroporous resin, and the yield was 85.38%. Conclusion HPD-300 macroporous resin is suitable for the purification of rutin, clovin, and coumarin in M. officinalis with high stability and good repeatability.