目的 考察參芪扶正注射液對(duì)環(huán)磷酰胺化療小鼠巨噬細(xì)胞吞噬功能和殺傷腫瘤細(xì)胞活性的影響。方法 將小鼠隨機(jī)分為對(duì)照組、環(huán)磷酰胺組以及參芪扶正注射液低、中、高劑量(10、20、40 mL/kg)組。除對(duì)照組外,其余各組小鼠均ip環(huán)磷酰胺100 mg/kg。參芪扶正注射液組小鼠ig給予10、20、40 mL/kg參芪扶正注射液,對(duì)照組和環(huán)磷酰胺組ig給予等量生理鹽水,1次/d,連續(xù)5 d。稱(chēng)定胸腺和脾臟質(zhì)量,計(jì)算免疫器官指數(shù);ELISA法檢測(cè)小鼠血清IL-2水平;流式細(xì)胞儀檢測(cè)巨噬細(xì)胞吞噬熒光微球的數(shù)量和比例,計(jì)算吞噬率和吞噬指數(shù);流式細(xì)胞儀檢測(cè)CFSE標(biāo)記的H22細(xì)胞中PI陽(yáng)性細(xì)胞的比例。結(jié)果 參芪扶正注射液可以不同程度地恢復(fù)環(huán)磷酰胺化療小鼠的胸腺指數(shù)和脾指數(shù);顯著提高環(huán)磷酰胺化療小鼠血清IL-2水平;減少小鼠巨噬細(xì)胞的吞噬率和吞噬指數(shù);不同程度地提高環(huán)磷酰胺化療后小鼠巨噬細(xì)胞對(duì)H22細(xì)胞的殺傷活性。結(jié)論 參芪扶正注射液可改善環(huán)磷酰胺化療后小鼠免疫抑制狀態(tài),對(duì)巨噬細(xì)胞吞噬和殺傷活性均有調(diào)節(jié)作用。

Objective To investigate the modulation effect of Shenqi Fuzheng Injection (SFI) on peritoneal macrophage phagocytosis and tumor-killing activity in cyclophosphamide (Cy) treated mice. Methods NIH mice were randomly divided into control group, Cy group, and low-, mid-, and high-dose (10, 20, and 40 mL/kg) SFI groups. Mice were ip administered with Cy (100 mg/kg) except control group. Mice in SFI groups were ig administrated with 10, 20, and 40 mL/kg SFI, once daily for 5 d, while those in control group and Cy group were ig administered with the same dosages of saline solutions. The weights of spleen and thymus were weighed to calculate the immune organ index. Levels of serum IL- 2 were determined by ELISA method. The amounts and proportions of macrophage phagocytosis of fluorescent microspheres were detected by Flow cytometry to calculate phagocytic rates and phagocytosis index. The proportion of PI positive cells in liver cancer cell H22 marked with CFSE was detected by Flow cytometry. Results SFI could recovery spleen and thymus index of Cy-treated mice in different extent, significantly improve the level of serum IL-2, and reduce phagocytic rates and phagocytosis index. SFI could also enhance the tumor-killing activity for liver cancer cell H22 of macrophages in Cy-treated mice in different degree. Conclusion SFI can improve immunosuppression, and regulate the phagocytosis function and enhance the tumor-killing activity of macrophages in Cy-treated mice.

高等學(xué)校博士學(xué)科點(diǎn)專(zhuān)項(xiàng)科研基金項(xiàng)目(20124425110010)