目的 探討當歸芍藥散水提取物對抗結(jié)核藥物肝損傷的保護作用及其機制。方法 將60只SD大鼠隨機分為6組,即對照組、模型組、硫普羅寧組(60 mg/kg)和當歸芍藥散水提取物低、中、高劑量(8、16、32 g/kg)組,給藥8周。分別測定大鼠血清丙氨酸氨基轉(zhuǎn)移酶(ALT)、天冬氨酸氨基轉(zhuǎn)移酶(AST)水平及血清和肝臟超氧化物歧化酶(SOD)、丙二醛(MDA)水平;采用RT-PCR方法測定大鼠肝臟腫瘤壞死因子-α(TNF-α)mRNA表達水平;利用Western Blotting法測定大鼠肝臟TNF-α蛋白表達水平。結(jié)果 與模型組比較,當歸芍藥散水提取物中、高劑量組均顯著降低血清ALT、AST水平,上調(diào)血清和肝臟SOD活性,下調(diào)血清和肝臟MDA水平,并降低肝臟TNF-α mRNA和蛋白表達水平。結(jié)論 當歸芍藥散水提取物對抗結(jié)核藥物肝損傷的保護作用機制可能與抑制TNF-α表達和脂質(zhì)過氧化有關(guān)。

Objective To investigate the protection of Danggui Shaoyao Powder water extracts on the hepatic injury induced by antituberculosis drugs in rats. Methods Sixty rats were divided randomly into six groups: Normal group, model group, tiopronin group (60 mg/kg), and Danggui Shaoyao Powder water extracts (8, 16, and 32 g/kg) groups. All groups were treated for 8 weeks. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, as well as the superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and liver were determined. Expression of hepatic tumor necrosis factor-α (TNF-α) mRNA was analyzed by RT-PCR method and its protein levels were assayed by Western Blotting method. Results Compared with model group, Danggui Shaoyao Powder water extracts at medium and high doses significantly reduced serum ALT and AST levels, elevated serum and liver SOD activities, down-regulated serum and liver MDA levels and reduced liver TNF-α mRNA and protein levels. Conclusion Danggui Shaoyao Powder water extracts has protective effects on the hepatic injury induced by antituberculosis drugs in rats which may be related to its inhibition of TNF-α and lipid peroxidation.

南京市醫(yī)學(xué)科技發(fā)展專項資金資助一般性課題(YKK12131)