目的 觀察丹參注射液預(yù)防大鼠急性胰腺炎及其對(duì)血清腫瘤壞死因子(TNF-α)、白細(xì)胞介素(IL-6)、胰腺組織核因子(NF-κB)及氧自由基的影響。方法 將60只SD大鼠隨機(jī)分為6組, 分別為假手術(shù)組、模型組、烏司他丁組(20 000 U/kg)以及丹參注射液低、中、高劑量(0.4、0.8、1.6 mL/kg)組。用5%?；悄懰徕c(1.0 mL/kg)胰管內(nèi)注入誘導(dǎo)大鼠急性胰腺炎模型, 造模前30 min分別尾iv給予丹參注射液。各組大鼠均于造模6 h后行腹主動(dòng)脈采血, 處死大鼠取胰腺組織標(biāo)本, 檢測(cè)SD大鼠急性胰腺炎模型血清淀粉酶(AMY)、血漿內(nèi)毒素(ET)、胰腺病理評(píng)分和病死率。并用ELISA法檢測(cè)血清IL-6、TNF-α濃度的變化, 運(yùn)用免疫組化法檢測(cè)NF-κ B表達(dá)水平, 同時(shí)切取各時(shí)間點(diǎn)大鼠胰腺組織, 檢測(cè)丙二醛(MDA)、組織髓過(guò)氧化物酶(MPO)及超氧化物歧化酶(SOD)的水平。結(jié)果 模型組大鼠的血清AMY、血漿ET、病理評(píng)分、死亡率、IL-6、TNF-α水平及胰腺組織中NF-κB活性、MDA水平和MPO活性均較假手術(shù)組升高(P<0.01), 而SOD活性則明顯降低(P<0.05);烏司他丁組和丹參注射液組上述各項(xiàng)指標(biāo)均較模型組明顯改善(P<0.05、0.01)。結(jié)論 丹參注射液可顯著降低實(shí)驗(yàn)性急性胰腺炎大鼠的IL-6、TNF-α及NF-κB, 清除氧自由基, 減輕脂質(zhì)過(guò)氧化反應(yīng), 從而減輕急性胰腺炎病理?yè)p害程度, 降低急性胰腺炎大鼠的死亡率。

Objective To investigate prevention of Danshen Injection on rats with acute pancreatitis, and determine the levels of TNF-α, IL-6, nuclear factor-κB (NF-κB) activity and oxygen free radicals. Methods Sixty SD rats were randomly divided into Sham group, model group, ulinastatin (20 000 U/kg) group and low-, mid-, and high-dose Danshen Injection (0.4, 0.8, and 1.6 mL/kg) groups. Acute pancreafitis model was developed by retrograde injection of 5% sodium taurocholate (1.0 mL/kg) into pancreatic duct in rats. Danshen Injection was injected into the tail vein 30 min before sodium taurocholate injection. The abdominal aortic blood and pancreatic specimens were taken 6 h after sodium taurocholate injection for measurement of serum amylase (AMY), plasma endotoxin (ET), pathological scores of pancreas and case-fatality rate. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) were determined by ELISA method. Expression of nuclear factor-κB (NF-κB) activity in pancreatic tissue was detected by immunohistochemical method, and oxygen free radicals, such as MDA, MPO, and SOD in each group were observed. Results The levels of serum AMY, plasma ET, pathological score versus, mortality, IL-6, TNF-α concentration, NF-κB levels of pancreas, pancreatic tissue, and the content of MDA and activity of MPO were enhanced (P<0.01), and the activity of SOD was significantly reduced in model group (P<0.05). Danshen Injection groups showed statistical improvement in plasma ET, serum AMY, pathological score, and oxygen free radicals compared with model group (P<0.05, 0.01). Conclusion Danshen Injection can significantly decrease NF-κB activity, IL-6, and TNF-αlevers, scavenge oxygen free radicals, and reduce lipid peroxidation, and therefore alleviate pancreatic damage in pancreas, leading to lowered level of case fatality in rats with acute pancreatitis.