目的 建立HPLC法測定病毒合劑中黃芩苷、綠原酸和連翹酯苷A的方法。方法 Shim-pack VP-ODS色譜柱（150 mm×4.6 mm，5 μm），流動相為0.5%磷酸水溶液–乙腈，采用梯度洗脫，檢測波長為300 nm，體積流量為1.0 mL/min，進樣量為10 μL。結(jié)果 黃芩苷、綠原酸和連翹酯苷A分別在9.84～82 μg/mL（r＝0.999 8）、2.64～22.0 μg/mL（r＝0.999 9）和4.32～36.0 μg/mL（r＝0.999 9）線性關(guān)系良好，平均回收率分別為99.94%、101.1%、99.77%，RSD值分別為1.44%、1.68%、1.44%（n=6）。結(jié)論 本法操作簡便、結(jié)果準確、重復性好，可用于病毒合劑的質(zhì)量控制。

Objective To establish an HPLC method for the determination of baicalin, chlorogenic acid, and forsythoside A in Bingdu Mixture. Methods HPLC was carried out on a Shim pack VP-ODS column (150 mm × 4.6 mm, 5 μm) with 0.5% phosphoric acid- acetonitrile as mobile phase for gradient elution. The detection wavelength was set at 300 nm. The injection volume was 10 μL at the flow rate of 1.0 mL/min. Results The liner ranges of baicalin, chlorogenic acid, and forsythoside A were 9.84 — 82 μg/mL (r = 0.999 8), 2.64 — 22.0 μg/mL (r = 0.999 9) and 4.32 — 36.0 μg/mL (r = 0.999 9); The average recoveries were 99.94%, 101.1%, and 99.77%, with RSD values of 1.44%, 1.68%, and 1.44% (n = 6), respectively. Conclusion The established method is simple, accurate, and reproducible and could be used for the quality control of Bingdu Mixture.