目的 研究長葉胡頹子乙醇提取物、三萜酸部位、熊果酸對4株腫瘤細(xì)胞增殖的體外抑制作用以及熊果酸抗腫瘤作用機(jī)制。方法 采用MTT法測定長葉胡頹子乙醇提取物、三萜酸部位和熊果酸在25、50、100、200 μg/mL時對人胃癌細(xì)胞SGC-7901、BGC-823、腸癌細(xì)胞LOVO、HCT-8實(shí)體瘤細(xì)胞增殖的體外抑制作用；并在上述藥物劑量下，觀察熊果酸作用72 h后對SGC-7901細(xì)胞形態(tài)的影響，以及作用24 h后對SGC-7901細(xì)胞凋亡的誘導(dǎo)作用。結(jié)果 長葉胡頹子乙醇提取物、三萜酸部位、熊果酸對4株腫瘤細(xì)胞的增殖具有較強(qiáng)的體外抑制作用，其中對于胃癌細(xì)胞的抑制作用為熊果酸＞三萜酸部位＞乙醇提取物；對腸癌細(xì)胞的抑制作用為三萜酸部位＞熊果酸＞乙醇提取物，同時熊果酸能顯著改變SGC-7901細(xì)胞的形態(tài)，并且SGC-7901細(xì)胞的凋亡率與熊果酸的劑量呈相關(guān)性。結(jié)論 三萜酸是長葉胡頹子發(fā)揮體外抗腸癌細(xì)胞增殖作用的主要有效部位；熊果酸是其發(fā)揮體外抗胃癌細(xì)胞增殖作用的主要活性成分，熊果酸抑制胃癌細(xì)胞增殖機(jī)制為誘導(dǎo)細(xì)胞凋亡，為開發(fā)新的抗癌藥物提供參考。

Objective To study the inhibitory effects of alcohol extracts, triterpenic acid fractions, ursolic acid from Elaeagnus bockii on the in vitro proliferation of four cancer cells, and the anticancer mechanism of ursolic acid. Methods The inhibitory effects of the alcohol extracts, triterpenic acid fractions and ursolic acid at the concentration of 25, 50, 100, and 200 μg/mL on human gastric cancer cells SGC-7901, BGC-823, colon cancer cells LOVO, HCT-8 were measured by MTT assay; SGC-7901 cell morphology was observed after treated by ursolic acid for 72 h, and the induction of SGC-7901 cell apoptosis was observed after treated by ursolic acid for 24 h. Results The alcohol extracts, triterpenic acid fractions, ursolic acid from E. bockii had the effective inhibitory effects on the in vitro proliferation of four cancer cells. The inhibitory rate of the alcohol extracts, triterpenic acid fractions, and ursolic acid on the proliferation of human gastric cancer cells was ursolic acid > triterpenic acid parts > alcohol extracts; The inhibitiory rate on the proliferation of human intestinal cancer cells was triterpenic acid parts > ursolic acid > alcohol extracts. Ursolic acid could significantly affect the morphology of SGC-7901 cell. The rate of apoptosis markedly elevated along with the increased concentration increasing of ursolic acid. Conclusion Triterpenic acid is the main active component on the proliferation of human intestinal cancer cell in vitro. The ursolic acid is the main anticancer effective component on the proliferation of gastric cancer cell in vitro. The anticancer mechanism of ursolic acid is inducing the cell apoptosis, and provides the theoretical basis for new anticancer drug.

湖北省衛(wèi)生廳科研基金項(xiàng)目（JX2C42）