目的 采用高效液相色譜法建立胡黃連總苷的特征圖譜。方法 Luna C18色譜柱（250 mm×4.6 mm，5 μm），流動(dòng)相為乙腈–0.5%冰醋酸水溶液（15.5∶84.5），體積流量為1.0 mL/min，柱溫為35 ℃，檢測(cè)波長(zhǎng)為275 nm，進(jìn)樣量為10 μL。結(jié)果 建立了胡黃連總苷的特征圖譜及其技術(shù)參數(shù)，即供試品特征圖譜中應(yīng)有11個(gè)特征峰，參照物峰相應(yīng)的峰為S峰，計(jì)算各特征峰與S峰的相對(duì)保留時(shí)間，其相對(duì)保留時(shí)間應(yīng)在規(guī)定值的±5%之內(nèi)。采用國(guó)家藥典委員會(huì)《中藥色譜指紋圖譜相似度評(píng)價(jià)系2004A版》自動(dòng)處理，10批胡黃連總苷的相似度均大于0.998。結(jié)論 本方法操作簡(jiǎn)便，準(zhǔn)確可靠，可用于控制胡黃連總苷的內(nèi)在質(zhì)量。

Objective To establish the characteristic chromatograms for the identification of the total glucosides in Picrorhiza scrophulariiflora (TGPS). Methods Methods The column was Luna Cl8 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile - 0.5% glacial acetic acid (15.5: 84.5), at the flow rate of 1.0 mL/min. The detection wavelength was 275 nm. The column temperature was 35 ℃ with the injection volume of 10 μL. Results Characteristic chromatograms and technial parameters of the TGPS were established. Eleven common peaks were separated in the test sample, and S peak was the reference peak. The relative retention time of each characteristic peak and S peak were calculated, and their relative retention time should be within ± 5% of the specified value. The similarity indexes of 10 batches were calculated by software published by the Chinese Pharmacopeia Committee. All these indexes were more than 0.998. Conclusion The method is simple, accurate and can be used for the quality control of the TGPS.

國(guó)家科技支撐計(jì)劃課題（2007BAI41B06）