目的 建立龍血竭的5種成分定量分析方法，為科學(xué)評(píng)價(jià)和有效控制龍血竭的質(zhì)量提供依據(jù)。方法 采用Phenomenex Kinetex C18柱（100 mm×4.6 mm，2.6 μm），以乙腈–水梯度洗脫，體積流量：1.7 mL/min，柱溫：40 ℃，檢測(cè)波長(zhǎng)為280 nm和325 nm。結(jié)果 白藜蘆醇、7,4′-二羥基黃酮、龍血素A、龍血素B和紫檀茋分別在7.76～248.4 μg/mL（r=0.999 9）、6.84～219 μg/mL（r=1.000 0）、5.75～184 μg/mL（r=1.000 0）、10.08～322.6 μg/mL（r=1.000 0）和23.71～758.6 μg/mL（r=0.999 8）呈良好的線性關(guān)系，平均回收率分別為97.61%、99.24%、102.72%、101.75%、102.65%，RSD值分別為1.16%、0.87%、0.86%、0.49%、1.10%。結(jié)論 本方法快速、簡(jiǎn)便、準(zhǔn)確，其結(jié)果可為龍血竭質(zhì)量標(biāo)準(zhǔn)的制定提供一定的依據(jù)。

Objective To establish a quantitative analysis method for the determination of multi-components in Draconis Resina, and to provide a evidence for the scientific evaluation and effective quality control of Draconis Resina. Methods Phenomenex Kinetex C18 column (100 mm × 4.6 mm, 2.6 μm) was used with a mobile phase of acetonitrile-water gradient elution. The flow rate was 1.7 mL/min, the column temperature was 40 ℃, and the detection wavelengths were 280 and 325 nm. Results The linear ranges of resveratrol, 7,4′-dihydroxy flavone, loureirin A, loureirin B, and pterostilbene were 7.76—248.4 μg/mL (r = 0.999 9), 6.84—219 μg/mL (r = 1.000 0), 5.75—184 μg/mL (r = 1.000 0), 0.08—322.6 μg/mL (r = 1.000 0), and 23.71—758.6 μg/mL (r = 0.999 8), respectively. The average recoveries of the five components were 97.61%, 99.24%, 102.72%, 101.75%, and 102.65% with RSD values of 1.16%, 0.87%, 0.86%, 0.49%, and 1.10%. Conclusion The method is rapid, simple, and accurate, and could be used for the quality control of Draconis Resina.