目的 篩選純化苦豆子多糖的最佳大孔樹脂并確定純化多糖的最佳工藝條件。方法 采用靜態(tài)吸附分離法對樹脂進(jìn)行篩選，并以苦豆子多糖的純度和回收率為考察指標(biāo)，對篩選出的樹脂進(jìn)行工藝優(yōu)化。結(jié)果 AB-8樹脂對苦豆子多糖有較好的吸附純化性能，最佳純化條件為：上樣多糖溶液質(zhì)量濃度為5 mg/mL，pH值為7.0，溫度為25 ℃，純化后的苦豆子多糖的質(zhì)量分?jǐn)?shù)達(dá)到88.90%，比純化前提高了17.60%，回收率90.62%。結(jié)論 AB-8 樹脂適用于苦豆子多糖的分離純化，該方法不僅穩(wěn)定有效，而且具有較高的多糖回收率。

Objective To optimize the macroporous resin and technologcial conditions for the purification of Sophora alopecuroides polysaccharides. Methods The static adsorption and separation method was used in the optimization of macroporous adsorption resin. The technological process was compared and evaluated in terms of the ratio of polysaccharide purity and coefficient of recovery. Results The resin AB-8 had the best performance of adsorption and desorption effects and was selected to purify S. alopecuroides polysaccharides. The optimum technology parameters were as follows: the sample concentration of 5 mg/mL, pH 7, and 25 ℃. The purity of polysaccharides was 88.90%, improved more 17.60% than that of crude polysaccharide, and the coefficient of recovery was 90.62% by using the above technology. Conclusion The resin AB-8 is a preferable candidate for the isolation and purification of S. alopecuroides polysaccharides, with the better stability and effectiveness and higher recovery.