目的 建立同時(shí)測定接骨片中阿魏酸和芍藥苷的高效液相色譜（HPLC）方法。方法 色譜柱為安捷倫Zorbax SB C18色譜柱（250 mm×4.6 mm，5 μm）；流動(dòng)相為乙腈–0.1%磷酸溶液（12∶88）；檢測波長為230 nm；體積流量為1.0 mL/min；柱溫為30 ℃；進(jìn)樣量為10 μL。結(jié)果 HPLC方法總運(yùn)行時(shí)間為20 min，阿魏酸和芍藥苷在線性范圍內(nèi)均呈現(xiàn)良好線性關(guān)系（r＞0.999 9），方法的精密度、重復(fù)性、加樣回收率均符合要求。結(jié)論 本法靈敏準(zhǔn)確、重復(fù)性好、操作簡便，可作為接骨片中阿魏酸和芍藥苷的測定及質(zhì)量控制方法。

Objective To establish a quantitative method for simultaneous determination of ferulic acid and paeoniflorin in Jiegu Tablets by HPLC. Methods The separation was performed on an Agilent Zorbax SB C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile and 0.1% phosphoric acid solution (12∶88), at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm. The column temperature was 30 ℃. The injection volume was 10 μL. Results The total running time was 20 min. The linear relationship between the concentration and peak areas of the two compounds were all linear (r >0.999 9). The precision, recovery, and repeatability tests met the demands for quantitative determination well. Conclusion The method could be used for the quality control of Jiegu Tablets with a good sensitivity, accuracy, and reproducibility.