目的 建立腺毛黑種草子中山柰酚的HPLC測定方法。方法 采用高效液相色譜法，色譜柱為Symmetry RP C18（150 mm×4.6 mm，5 μm）色譜柱，流動相為甲醇–0.4%磷酸水溶液（70∶30），體積流量1.0 mL/min，檢測波長365 nm，進(jìn)樣量10 μL。結(jié)果 山柰酚在0.022 4～0.134 4 μg呈良好的線性關(guān)系（r=0.999 9），平均回收率為97.59%，RSD值為1.29%（n=9）。結(jié)論 該方法操作簡便、準(zhǔn)確、重復(fù)性好，可用于腺毛黑種草子中山柰酚的測定。

Objective To establish an HPLC method for determination of kaempferol in the seeds of Nigella glandulifera. Methods HPLC was carried out on Symmetry C18 column (150 mm×4.6 mm, 5 μm) with methanol-0.4% phosphoric acid (70∶30) as mobile phase. The detection wavelength was 365 nm and injection volume was10 μL at the flow rate of 1.0 mL/min. Results The calibration curve was linear over the range of 0.022 4—0.134 4 μg (r=0.999 9) for kaempferol. The average recovery rate was 97.59% with RSD value at 1.29%. Conclusion The established method is rapid, sensitive, and accurate, and could be used for the determination of kaempferol in the seeds of N. glandulifera.

新疆維吾爾自治區(qū)科技攻關(guān)和重點(diǎn)科技項(xiàng)目（200433115）