目的 建立靈芝子實(shí)體和孢子粉中3種靈芝酸類成分的HPLC測定方法，為全面評價(jià)靈芝藥材和孢子粉的質(zhì)量提供依據(jù)。方法 采用HPLC方法測定，Diamonsil（鉆石）C8色譜柱（250 mm×4.6 mm，5 μm），流動(dòng)相乙腈–0.03%磷酸（28∶72），體積流量1.2 mL/min，檢測波長250 nm；柱溫35 ℃。結(jié)果 靈芝酸C2、靈芝酸G、靈芝酸A分別在0.40～10.06 μg（r=0.999 99）、0.40～10.00 μg（r=0.999 99）、0.40～10.00 μg（r=0.999 99）線性關(guān)系良好，平均回收率分別為101.04%、99.39%、101.22%，RSD值分別為1.64%、1.86%、2.20%（n=6）；靈芝子實(shí)體中3種靈芝酸的質(zhì)量分?jǐn)?shù)分別在0.06～0.29 mg/g、0.12～0.37 mg/g、0.19～0.41 mg/g，靈芝孢子粉供試品中3種靈芝酸的質(zhì)量分?jǐn)?shù)分別在0～0.01 mg/g、0、0～0.03 mg/g。結(jié)論 本方法簡便，穩(wěn)定，可用于靈芝三萜類成分C2、靈芝酸G、靈芝酸A的定量分析。

Objective To provide the scientific evidence for overall quality evaluation of Ganoderma, a trial to develop HPLC method for quantitative analysis of three ganoderic acids in Ganoderma was carried out. Methods The HPLC method was performed on Diamonsil C8 column (250 mm×4.6 mm, 5 μm), with acetonitrile-0.03% phospholic acid (28∶72) as the mobile phase; The flow rate was 1.2 mL/min; UV detection wavelength was 250 nm; The column temperature was 35 ℃. Results The liner ranges of ganoderic acid C2, ganoderic acid G and ganoderic acid A were 0.40—10.06 μg (r=0.999 99), 0.40—10.00 μg (r=0.999 99) and 0.40—10.00 μg (r=0.999 99); The average recoveries were 101.04%, 99.39% and 101.22%, with RSD values of 1.64%, 1.86%, and 2.20% (n=6), respectively. The content ranges of three ganoderic acids in sporophore were 0.06—0.29 mg/g, 0.12—0.37 mg/g, and 0.19—0.41 mg/g, respectively, and those in spore powder were 0—0.01 mg/g, 0, and 0—0.03 mg/g, respectively. Conclusion The method is proved to be convenient and reliable, so it could be used for the quantitative analysis of ganoderic acids in Ganoderma.

國家重大新藥創(chuàng)制科技專項(xiàng)（2011ZX09401-009）