目的 觀察散結(jié)鎮(zhèn)痛片對子宮內(nèi)膜異位癥模型大鼠妊娠功能和雌激素水平的影響。方法 采用自體種植法制備大鼠子宮內(nèi)膜異位癥模型。手術(shù)后將大鼠分為假手術(shù)組，模型組，達(dá)那唑膠囊（0.070 g/kg）陽性對照組，散結(jié)鎮(zhèn)痛片低、中、高劑量（0.216、0.432、0.864 g/kg）組及散結(jié)鎮(zhèn)痛膠囊（0.864 g生藥/kg）組，每天ig給藥1次，連給4周后，大鼠眼眶靜脈叢取血，放射免疫法測血清中促黃體生成素、催乳素和孕激素的水平。取各組大鼠與有生育能力的雄鼠合籠交配，1周后將雄鼠取出，分籠飼養(yǎng)，于分籠后第12天處死雌鼠，摘取右卵巢，計數(shù)妊娠黃體數(shù)，剖開右宮角，計數(shù)著床點(diǎn)、活胎及死胎數(shù)，并計算著床率和死胎率。結(jié)果 與模型組比較，散結(jié)鎮(zhèn)痛片3個劑量均能增加活胎數(shù)、排卵數(shù)，提高著床率，降低死胎率，其中高劑量增加排卵數(shù)，提高著床率的作用尤為明顯；散結(jié)鎮(zhèn)痛片3個劑量均能調(diào)節(jié)大鼠血清催乳素和孕激素水平，其中高劑量降低催乳素、升高孕激素水平的作用尤為明顯。結(jié)論 散結(jié)鎮(zhèn)痛片能增強(qiáng)子宮內(nèi)膜異位癥模型大鼠的妊娠功能，該作用可能與其調(diào)節(jié)激素水平、恢復(fù)機(jī)體正常內(nèi)分泌功能有關(guān)。

Abstract: Objective To observe the effects of Sanjie Zhentong Tablet on pregnant function and estrogen level of rats with experimental endometriosis. Methods Self-endometrium transplantation by surgical method was used to develop rats models of endometriosis. After operation, the rats were divided into seven groups, such as Sham group, model group, Danazuo (0.070 g/kg) group, high, medium, and low dosage (0.216、0.432、0.864 g/kg) of Sanjie Zhentong Tablet group, and Sanjie Zhentong Capsula (crude drugs 0.864 g/kg) group. Four weeks after ig administration once daily, blood samples were drawn from orbital venous plexus of rats, the serum LH, P and PRL levels were measured by radioimmunoassay. Then the rats were mated with known fertile male rats, that were removed after one week, sub-cage feeding, 12 d later, females were killed to open the abdominal cavity, then remove the right ovary, count the number of corpus luteum of pregnancy, cut and open the right uterine horn, count the implantation sites, the number of live births and stillbirths, and calculate the implantation rate and stillbirth rate. Results Three doses of Sanjie Zhentong Tablet can increase the number of live births, ovulation rate, and the implantation rate, while reduce the stillbirth rate, among which high dose of Sanjie Zhentong Tablet could significantly increase the number of ovulation rate and implantation rate；all the three doses of Sanjie Zhentong Tablet are able to regulate the level of serum PRL and P, in which large doses can significantly reduce the serum levels of PRL, elevate P level. Conclusion Sanjie Zhentong Tablet can improve the pregnant functions of experimental endometriosis in rats, the mechanism may be related to its functions in regulating hormone levels and restoring the body's normal endocrine.

國家重大新藥創(chuàng)制專項(xiàng)（2011ZX092012-066）