目的 建立一種HPLC法快速測(cè)定北冬蟲(chóng)夏草和幾種常見(jiàn)食用菌中腺苷和蟲(chóng)草素的方法。方法 Welch Material XB-C18色譜柱（250 mm×4.6 mm， 5 μm），以水–甲醇（85︰15）為流動(dòng)相，體積流量1.0 mL/min，檢測(cè)波長(zhǎng)258 nm，柱溫40 ℃。結(jié)果 腺苷在0.023 2～0.464 0 μg、蟲(chóng)草素在0.030 0～0.600 0 μg與峰面積線性關(guān)系良好，平均加樣回收率分別為98.97%、99.15%，RSD分別為1.41%、1.01%。結(jié)論 腺苷普遍存在于各種食用菌子實(shí)體中，而蟲(chóng)草素是蟲(chóng)草屬真菌子實(shí)體中的特有活性成分。本法簡(jiǎn)便快速，為全面評(píng)估北冬蟲(chóng)夏草中核苷類成分的質(zhì)量，推進(jìn)人工培育北冬蟲(chóng)夏草替代野生冬蟲(chóng)夏草提供檢測(cè)方法。

Objective To develop a rapid method by using HPLC to quantitatively determine adenosine and cordycepin in Cordyceps militaris products and common edible fungi. Methods The working conditions: column was Welch Material XB-C18 column (250 mm×4.6 mm, 5 μm), mobile phase was composed of water-methanol (85︰15) with flow rate of 1.0 mL/min and 258 nm detection wavelength, and temperature was 40 ℃. Results The linearity range of adenosine and cordycepin were 0.023 2—0.464 0 and 0.030—0.600 μg. The average recovery of adenosine and cordycepin were 98.97% and 99.15% with the RSD of 1.41% and 1.01%, respectively. Conclusion Adenosine appears widely in common edible fungi, and cordycepin is characteristic in fruitbodies of Cordyceps (Fr.) Link. The HPLC method is simple and fast., and could lay a method on the comprehensive evaluation of the nucleoside components in C. militaris, and promot the cultured C. militaris to replace the natural C. sinensis resources.

國(guó)家863計(jì)劃重點(diǎn)項(xiàng)目（2007AA021502）