摘 要：目的 建立高效液相色譜法測定米諾膦酸片中米諾膦酸。方法 采用安捷倫XDB-C18色譜柱（250 mm×4.6 mm，5 μm），流動相為0.03 mol/L焦磷酸鈉水溶液（含四丁基溴化銨0.322 g/L，以磷酸調節(jié)pH 7.5）-甲醇（85∶20），體積流量1 mL/min，柱溫30 ℃，檢測波長280 nm，進樣量20 μL。結果 米諾膦酸在0.02～0.18 mg/mL時，峰面積與質量濃度呈良好的線性關系（r=0.999 9）。結論 該方法準確、可靠，可作為米諾膦酸片的測定方法。

Abstract: Objective To establish a RP-HPLC method for the determination of minodronic acid in Minodronic Acid Tablets. Method The Agilent column XDB-C18 (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisited of 0.03 mol/L sodium pyrophosphate (0.322 g/L tetrabutylammonium bromide, adjusting pH value to 7.5 with phosphorous acid)-methanol (85∶20). The flow rate was 1.0 mL/min with the column temperature at 30 ℃. The detection wavelenth was 280 nm and the injection volumn was 20 μL. Results The solution of minodronic acid was stability. The linear range for minodronic acid was 0.02—0.18 mg/mL (r=0.999 9). Conclusion The method is accurate and reliable, which is suitable for the determination of Minodronic Acid Tablets.