摘 要：目的 研究西黃丸中具有抑制血管生成活性的組方藥味。方法 采用發(fā)育時(shí)間為24 h的血管熒光標(biāo)記轉(zhuǎn)基因斑馬魚胚胎作為模型，熒光顯微鏡下觀察50 μg/mL西黃丸甲醇提取物及其組方藥味人工牛黃、人工麝香、乳香和沒藥甲醇提取物20 μg/mL給藥24 h后對(duì)斑馬魚節(jié)間血管（IV）形成的影響。結(jié)果 人工麝香、沒藥提取物對(duì)斑馬魚IV數(shù)量無影響，人工牛黃組IV數(shù)為22.67±2.60，抑制率為17.3%；乳香提取物組IV數(shù)分別為17.2±5.71，抑制率為37%；與對(duì)照組比較，人工牛黃、乳香提取物具有顯著的抑制斑馬魚IV生成的作用（P＜0.05或0.01）。結(jié)論 乳香與人工牛黃是西黃丸中具有抑制血管生成作用的主要藥味。

Abstract: Objective To study the component herbs for angiogenesis inhibition in Xihuang Pills. Methods Using 24 h vascular fluorescent transgenic zebrafish embryos as model, with fluorescent microscope the effects of 50 μg/mL Xihuang Pills' methanol extracts and four herbs methanol extracts which are artificial Bovis Calculus, artificial moschus, frankincense, and myrrh methanol (20 μg/mL) on the zebrafish intersegmental vessels (IV) angiogenesis were observed in administration of 24 h.. Results Extract of artificial musk and myrrh had no effect on zebrafish IV angiogenesis, IV number in artificial Bovis Calculus group was 22.67 ± 2.60, inhibition rates was 17.3%; IV number in frankincense group were 17.2 ± 5.71, inhibition rates was 37%. Artificial Bovis Calculus and frankincense could inhibit zebrafish IV angiogenesis significantly (P＜0.05 or 0.01) compared with the non-treated group. Conclusion The frankincense and artificial Bovis Calculus are main herbs in the Xihuang Pills that could inhibit angiogenesis.

山東省科學(xué)院科技發(fā)展基金項(xiàng)目（SY2007BS10）