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Archive > Volume 17 Issue 3 > 2025,17(3):555-561. DOI:https://doi.org/10.1016/j.chmed.2024.08.001 Prev Next

DNA條形碼在快速擴(kuò)散居群中的應(yīng)用和挑戰(zhàn)：以景天科紅景天屬為例

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Applications and challenges of DNA barcoding in rapid radiation groups: Rhodiola (Crassulaceae) as a case study

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    摘要:
    目的：紅景天藥材，是大花紅景天的根及根莖，它在醫(yī)藥市場(chǎng)上的應(yīng)用非?；靵y。本研究建立了紅景天屬物種的條形碼數(shù)據(jù)庫(kù)和識(shí)別引擎，對(duì)來(lái)自藥材市場(chǎng)的飲片進(jìn)行了鑒定，并分析了DNA條形碼在紅景天屬快速輻射中的應(yīng)用和挑戰(zhàn)。本研究為紅景天屬瀕危資源的保護(hù)和合理開(kāi)發(fā)利用提供參考。 方法：我們采集了河北、新疆、西藏、吉林等主產(chǎn)區(qū)20種紅景天屬50份原植物樣品。建立了紅景天屬I(mǎi)TS2和psbA-trnH條形碼數(shù)據(jù)庫(kù)，并開(kāi)發(fā)了一個(gè)名為Rhodiola-IDE的識(shí)別引擎。使用兩個(gè)數(shù)據(jù)集評(píng)估標(biāo)準(zhǔn)DNA條形碼數(shù)據(jù)庫(kù)的穩(wěn)定性和準(zhǔn)確性。Rhodiola-IDE鑒定了來(lái)自藥材市場(chǎng)的31份飲片樣品。 結(jié)果：建立了含有88種紅景天1532個(gè)序列的條形碼數(shù)據(jù)庫(kù)，其鑒定結(jié)果具有良好的準(zhǔn)確性和穩(wěn)定性。根據(jù)《中國(guó)藥典》（2020年版），21個(gè)樣品（67.7%）被鑒定為正品（大花紅景天），10個(gè)樣品（32.3%）被認(rèn)定為偽品。 結(jié)論：ITS2和psbA-trnH條形碼能夠鑒定以大花紅景天為代表的單系群。然而，對(duì)于非單系物種，有必要收集盡可能多的樣本，并將其與多個(gè)標(biāo)記結(jié)合進(jìn)行鑒定。探討了快速輻射條件下DNA條形碼在紅景天屬中的應(yīng)用及其面臨的挑戰(zhàn)，為紅景天品種的合理開(kāi)發(fā)利用提供了科學(xué)依據(jù)。

    Abstract:
    Objective: Rhodiolae Crenulatae Radix et Rhizoma (Hongjingtian in Chinese, RCRR), the roots and rhizomes of Rhodiola crenulata and its application in the medicinal market is very chaotic. In this study, DNA barcoding database and identification engine of Rhodiola species were established, decoction pieces from the medicinal market were identified, and the application and challenges of DNA barcoding in the rapid radiation of Rhodiola species were analyzed. This study provides reference for the protection, rational development, and utilization of endangered resources within Rhodiola species. Methods: A total of 50 original plant samples from 20 species of the genus Rhodiola from Hebei, Xinjiang, Tibet, Jilin, and other major production areas were collected. Theses samples cover the typical distribution area (Qinghai-Tibetan Platea) of Rhodiola species and other scattered alpine regions (Changbai Mountain, Taibai Mountain, Lushan Mountain, etc.), it encompasses all Rhodiola species with thick rhizomes in China. ITS2 and psbA-trnH barcode of Rhodiola database (BORD) were established and an identification engine named Rhodiola-IDE was developed. The stability and accuracy of the standard DNA barcoding database were evaluated using two datasets. Rhodiola-IDE identified 31 decoction pieces of RCRR from the medicinal material market. Results: The BORD containing 1 532 sequences of 88 Rhodiola species has been established, and the identification efficiency results showed good accuracy and stability. According to the Chinese Pharmacopoeia (2020 edition), 23 samples (74.2%) were identified as authentic R. crenulata, while the rest of the marketed varieties were R. kirilowii, R. dumulosa, and R. fastigiata. The product label ‘‘Larger flower, Hongjingtian” was identified as R. crenulata. Samples labeled as ‘‘Smaller flower, Hongjingtian” were identified as R. crenulata, R. kirilowii, and R. fastigiata. Conclusion: ITS2 and psbA-trnH barcodes can identify monophyletic groups represented by R. crenulata. However, for non-monophyletic species, it is necessary to collect as many samples as possible and combine them with multiple markers for joint identification. This study discussed the application and challenges of DNA barcodes in Rhodiola under rapid radiation conditions, providing a scientific basis for the rational development and utilization of Rhodiola varieties.

    關(guān)鍵詞:
    大花紅景天;紅景天屬;紅景天;DNA條形碼;ITS2;psbA-trnH;快速擴(kuò)散

    Keywords:


    Project Supported:
    This study was supported by China Postdoctoral Science Foundation (No. 2022M720504), the National Key Research and Development Program of China: Intergovernmental Cooperation in International Science and Technology Innovation (No. 2022YFE0119300), CAMS Innovation Fund for Medical Sciences (CIFMS) (No. 2021-I2M-1-029 and No. 2021-I2M-1-032), Open project supported by Hebei (Chengde) Industrial Technology Institute of Chinese Medicinal Materials (No. CYKF202301), the graduate student innovation ability training of Education of Hebei Province Department (No. CXZZSS2024118), and Key project at central government level: The ability establishment of sustainable use for valuable Chinese medicine resources (No. 2060302).

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