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Archive > Volume 17 Issue 3 > 2025,17(3):513-528. DOI:https://doi.org/10.1016/j.chmed.2025.03.006 Prev Next

核黃素代謝參與的野生黃芪與平栽黃芪對慢性萎縮性胃炎的治療作用比較研究

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Riboflavin metabolism involved into comparison of therapeutic action of wild-simulated and transplanted Astragali Radix to treat chronic atrophic gastritis rats based on endogenous-xenobiotics metabolism

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    摘要:
    目的：黃芪（Astragali Radix，AR）在中國廣泛用作治療慢性萎縮性胃炎（chronic atrophic gastritis，CAG）。仿野生黃芪（WAR）和平栽黃芪（TAR）是兩種最常購買的品種。然而，它們的療效尚不清楚。本研究旨在通過對比兩種AR在抗CAG中的作用，進一步明確它們的療效差異。 方法：采用超高效液相色譜-四極桿飛行時間質(zhì)譜（UHPLC-Q-TOF/MS）進行代謝組學(xué)分析，研究了這兩種AR在治療CAG時盲腸內(nèi)容物中的代謝特征和代謝過程的差異。 結(jié)果：表觀藥效指標(biāo)顯示，WAR具有更顯著的治療效果。代謝組學(xué)結(jié)果表明，在兩種黃芪干預(yù)后，大鼠有7種內(nèi)源代謝物存在顯著差異。此外，在WAR組和TAR組治療后，在盲腸內(nèi)容物中分別鑒定出77種和65種外源代謝物。最后，我們選擇了在慢性萎縮性胃炎形成中最關(guān)鍵的核黃素代謝途徑，與相關(guān)的外源成分進行分子對接，結(jié)果顯示W(wǎng)AR的得分高于TAR。 結(jié)論：在本研究中，我們結(jié)合代謝組學(xué)和藥物化學(xué)技術(shù)，分析了不同種植形式的黃芪的療效差異，為中藥療效評估和質(zhì)量控制研究提供了新的視角。

    Abstract:
    Objective: Astragali Radix (AR, Huangqi in Chinese) has been utilized generally as a bulk drug for the treatment of chronic atrophic gastritis (CAG) in China. The efficacy of wild-simulated AR (WAR) and transplanted AR (TAR) commercially available is unclear. This study aimed to further clarify the therapeutic action of WAR and TAR to treat CAG rats based on endogenous-xenobiotics metabolism. Methods: Ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometer (UHPLC-Q-TOF/MS) based metabolomics had been used to analyze the cecal contents metabolic features and metabolic process differences of two ARs in the treatment of CAG. Results: Apparent pharmacodynamic indicator examination revealed that the WAR group had a more substantial curative effect. Metabolomics studies revealed that seven endogenous metabolites were significant differences in two ARs. Furthermore, following treatment, 77 and 65 xenobiotics metabolites were identified in the WAR and TAR groups, respectively. Finally, the most critical riboflavin metabolic route in the formation of CAG was chosen for molecular docking with the relevant exogenous components, and WAR scored higher than TAR. Conclusion: In this work, we analyzed the efficacy differences of AR from diverse cultivation forms by combining metabolomics and medicinal chemistry technologies, and it gave a fresh perspective for TCM efficacy evaluation and quality control research.

    關(guān)鍵詞:
    黃芪;慢性萎縮性胃炎;內(nèi)源性代謝物;核黃素;平栽黃芪;仿野生黃芪;外源性代謝物

    Keywords:


    Project Supported:
    This work was supported by National Natural Science Foundation of China (No. 82374025 and U23A20517).

    Clc Number:

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