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Archive > Volume 16 Issue 3 > 2024,16(3):466-480. DOI:https://doi.org/10.1016/j.chmed.2024.01.006 Prev Next

UPLC-Q-TOF/MS法鑒定和表征麻黃桂枝化學成分及其在大鼠血漿和腦組織中的代謝產(chǎn)物

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Identification and characterization of chemical constituents in Mahuang Guizhi Decoction and their metabolites in rat plasma and brain by UPLC-Q-TOF/MS

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    摘要:
    目的：麻黃桂枝是中國傳統(tǒng)醫(yī)學中的重要藥對，具有解表散寒，清熱平喘的功效，主要含有生物堿類、黃酮類、苯丙素類和氨基酸類四類化合物。 方法：在本研究中，我們采用UPLC-Q-TOF-MS法鑒定麻黃桂枝湯中的化學成分及其原型成分在血漿和腦組織中吸收的代謝產(chǎn)物。繪制了麻黃桂枝湯原型成分和代謝產(chǎn)物在血漿和腦組織中的藥時曲線。 結(jié)果：我們鑒定出麻黃桂枝湯中105個化學成份，其中30個可吸收入學，10個可吸收入腦。在血漿中發(fā)現(xiàn)8個新的生物轉(zhuǎn)化代謝產(chǎn)物，其中有一半可以通過血腦屏障。此外，在體內(nèi)檢測到的所有成分可以立即被吸收和分布。 結(jié)論：這些發(fā)現(xiàn)為分析麻黃桂枝湯中潛在的生物活性成分及其體內(nèi)過程提供了一種可行的研究方法，可促進麻黃桂枝湯藥效物質(zhì)基礎和臨床應用的安全性研究。

    Abstract:
    Objective: Mahuang Guizhi Decoction (MGD), an essential herbal pair in traditional Chinese medicine, is able to release cold, fever and asthma, mainly containing alkaloids, flavonoids, phenylpropanoids and amino acids. However, the absorption and distribution of these four category compounds in vivo still remained unclearly. Methods: In our research, we utilized UPLC-Q-TOF-MS technique to identify the constituents within MGD, as well as the prototypes of MGD and their metabolites absorbed in plasma and brain. We further profiled the drug-time curve of prototypes and metabolites of MGD both in plasma and brain. Results: Our results showed that 105 constituents were characterized in MGD. Thirty of them could be absorbed into blood, and ten of them could be distributed into brain. We also discovered eight new bio-transformed metabolites in blood, and a half of which could pass through the blood–brain barrier. In addition, all components detected in vivo could be absorbed and distributed immediately. Conclusion: These findings provide an approachable method to analyze the potential bio-active compounds in MGD and their in vivo behaviors, which could promote the efficacious material basis study of MGD and the security of clinical utilization.

    關鍵詞:
    化學成分 ;麻黃桂枝湯;代謝產(chǎn)物;大鼠血漿和腦;UPLC-Q-TOF-MS

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    Project Supported:
    This work was supported by Shenzhen Science and Technology Program (No. JCYJ20210324140204011), Shenzhen Science and Technology Program (No. JSGG20210802093208023), Shenzhen Science and Technology Program (No. ZDSYS201606081515458), Foundation of Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare Products (No. GPKLFSHP202101), and the Sanming Project of Medicine in Shenzhen (No. SZZYSM202111002).

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