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Archive > Volume 16 Issue 3 > 2024,16(3):435-448. DOI:https://doi.org/10.1016/j.chmed.2023.10.004 Prev Next

代謝組和轉(zhuǎn)錄組的綜合分析為中藥百合的功能成分提供了新的見解

Original articles

Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus

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    摘要:
    目的：百合（Lilium brownii）和卷丹（L. lancifolium）是我國中藥百合的主要來源。然而，這兩種百合治療疾病的藥效物質(zhì)基礎(chǔ)尚不清楚。我們選擇了僅作為食用的蘭州百合作為空白對照組，通過代謝組學(xué)和轉(zhuǎn)錄組學(xué)分析蘭州百合與百合，蘭州百合與卷丹的差別，進一步探究中藥百合的藥效物質(zhì)基礎(chǔ)。 方法：通過代謝組學(xué)和轉(zhuǎn)錄組學(xué)分析蘭州百合與百合，蘭州百合與卷丹的差異。 結(jié)果：代謝組學(xué)主成分分析和聚類熱圖分析表明卷丹與蘭州百合、百合與蘭州百合的代謝物分離且差異較大。分別有318和298個顯著性差異代謝物存在于蘭州百合vs百合、蘭州百合vs卷丹組中。與蘭州百合相比，百合和卷丹中的甾體皂苷和甾體生物堿均顯著上調(diào)，尤其是百合中。轉(zhuǎn)錄組學(xué)聚類熱圖分析表明，百合與蘭州百合之間存在顯著差異，而卷丹和蘭州百合之間的差異較小。此外，在蘭州百合vs百合組中鑒定了47540個差異表達基因，在蘭州百合vs卷丹組中鑒定出18958個差異表達基因。值得注意的是，檢測到參與甾體皂苷和甾體生物堿生物合成的CYP450，與蘭州百合相比，百合中的CYP724、CYP710A和CYP734A1以及卷丹中的CYP90B均上調(diào)。 結(jié)論：本研究表明，甾體皂苷和甾體生物堿可能是中藥百合的代表性功能成分，可為中藥百合在經(jīng)典名方研發(fā)中的應(yīng)用提供了新的思路。

    Abstract:
    Objective: Lilium brownii var. viridulum (LB) and L. lancifolium (LL) are the main sources of medicinal lily (Lilii Bulbus, Baihe in Chinese) in China. However, the functional components of these two species responsible for the treatment efficacy are yet not clear. In order to explore the therapeutic material basis of Lilii Bulbus, we selected L. davidii var. willmottiae (LD) only used for food as the control group to analyze the differences between LD and the other two (LB and LL). Methods: Metabolome and transcriptome were carried out to investigate the differences of active components in LD vs LB and LD vs LL. Data of metabolome and transcriptome was analysed using various analysis methods, such as principal component analysis (PCA), hierarchical cluster analysis (HCA), and so on. Differentially expressed genes (DEGs) were enriched through KEGG and GO enrichment analysis. Results: The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD. There were 318 significantly differential metabolites (SDMs) in LD vs LB group and 298 SDMs in LD vs LL group. Compared with LD group, the significant upregulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups, especially in LB group. The HCA of transcriptome indicated that there was significant difference in LB vs LD group, while the difference between LL and LD varied slightly. Additionally, 47 540 DEGs in LD vs LB group and 18 958 DEGs in LD vs LL group were identified. Notably, CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected, and comparing with LD, CYP724, CYP710A, and CYP734A1 in LB and CYP90B in LL were all up-regulated. Conclusion: This study suggested that steroidal saponins and steroidal alkaloids maybe the representative functional components of Lilii Bulbus, which can provide new insights for Lilii Bulbus used in the research and development of classic famous formula.

    關(guān)鍵詞:
    百合;卷丹;蘭州百合;代謝物;轉(zhuǎn)錄組

    Keywords:


    Project Supported:
    This research was funded by 2022 Provincial Science and Technology Research and Development Plan United Fund (No. 222301420075), Henan Provincial High-Level Talents International Training Funding Project (No. 2021-72).

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