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Archive > Volume 16 Issue 2 > 2024,16(2):274-281. DOI:https://doi.org/10.1016/j.chmed.2023.10.003 Prev Next

黃芪桂枝五物湯通過抑制炎癥和骨破損改善膠原誘導性關(guān)節(jié)炎小鼠模型癥狀的研究

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Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

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    摘要:
    目的：類風濕關(guān)節(jié)炎（RA）是一種慢性炎性和破壞性關(guān)節(jié)炎，其特征在于炎性浸潤和骨破損。黃芪桂枝五物湯（HGWD）是中國傳統(tǒng)中藥復(fù)方，在臨床上被應(yīng)用于RA的治療。本研究旨在探究HGWD對膠原誘導性關(guān)節(jié)炎（CIA）小鼠模型的治療效果。 方法：采用雌性DBA/1J小鼠建立膠原誘導關(guān)節(jié)炎模型。第一次免疫后第22天開始每天一次HGWD灌胃給藥，持續(xù)四周。每五天測量小鼠體重、后爪厚度和臨床癥狀評分。進行步態(tài)分析、組織病理學染色、酶聯(lián)免疫吸附測定（ELISA）、超聲成像和顯微計算機斷層成像以確定該模型中HGWD治療對炎癥和骨結(jié)構(gòu)的影響。 此外，通過RT-PCR和Western blot檢測HGWD干預(yù)對Raw 264.7細胞相關(guān)炎癥因子mRNA及蛋白質(zhì)水平的表達情況。 結(jié)果：HGWD可以減輕CIA小鼠關(guān)節(jié)炎癥狀，抑制炎癥滑膜面積和血清中炎癥因子的水平，抑制膝關(guān)節(jié)和踝關(guān)節(jié)的關(guān)節(jié)空間增大，減少破骨細胞數(shù)量，保護骨破壞，以及改善運動功。同時HGWD降低可以降低LPS誘導后Raw 264.7炎癥因子mRNA的表達和p-NF-κb和IL-17的蛋白質(zhì)表達水平。 結(jié)論：這些結(jié)果表明HGWD具有抑制炎癥，減少骨侵蝕和保持運動功能的效果。

    Abstract:
    Objective: Rheumatoid arthritis (RA) is a chronic inflammatory and destructive arthritis, characterized by inflammatory infiltration and bone destruction. Huangqi Guizhi Wuwu Decoction (HGWD) is traditional Chinese medicine, which has been applied in the treatment of RA in clinical. The aim of this study was to investigate the therapeutic effect of HGWD on collagen-induced arthritis (CIA) mouse model. Methods: DBA/1J female mice were used to establish the collagen-induced arthritis (CIA) model. HGWD was administered intragastrically once a day for four weeks starting on the 22nd day after the first immunization. The body weight, hind paw thickness and clinical score were measured every five days. Gait analysis, histopathological staining, enzyme-linked immunosorbent assay (ELISA), ultrasound imaging and micro-computed tomography imaging were performed to determine the effects of HGWD treatment on inflammation and bone structure in this model. Moreover, Real-time PCR and Western blot analysis were used to detect inflammatory factors mRNA and protein levels after HGWD intervention in RAW 264.7 cells. Results: HGWD attenuated symptoms of arthritis, suppressed inflammatory synovium area and the serum levels of inflammatory factors, inhibited joint space enlargement in the knee and ankle joints, reduced numbers of osteoclasts, protected bone destruction, as well as improved motor function. HGWD decreased the expression of mRNA for inflammatory factors and the protein expression levels of p-NF-κB and IL-17. Conclusion: These results suggested that HGWD suppresses inflammation, attenuates bone erosion and maintains motor function in collagen-induced arthritis mice.

    關(guān)鍵詞:
    骨破損;膠原誘導性關(guān)節(jié)炎;黃芪桂枝五物湯;炎癥;類風濕關(guān)節(jié)炎

    Keywords:


    Project Supported:
    This work was sponsored by National Natural Science Foundation (No. 81822050, 81920108032, 81904227), Shanghai ‘‘Science and Technology Innovation Action Plan” Medical Innovation Research Project (No. 21Y11921400), the Program for Innovative Research Team of Ministry of Science and Technology of China (No. 2015RA4002), ‘‘Innovation Team” Development Projects (No. IRT1270), Innovative Team Project of Scientific Research Project of Traditional Chinese Medicine of Shanghai Municipal Health Commission (No. 2022CX001), Shanghai TCM Medical Center of Chronic Disease (No. 2022ZZ01009), Jing’an District Health Research Project of Shanghai (No. 2022MS03).

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