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Archive > Volume 16 Issue 2 > 2024,16(2):235-238. DOI:https://doi.org/10.1016/j.chmed.2023.07.004 Prev Next

夾竹桃葉內(nèi)生真菌炭疽菌HK-08來(lái)源的新的吲哚衍生物

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New indole derivatives from endophytic fungus Colletotruchum sp. HK-08 originated from leaves of Nerium indicum

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    摘要:
    目的：為了研究夾竹桃葉內(nèi)生真菌炭疽菌HK-08的次生代謝產(chǎn)物。 方法：通過(guò)各種柱色譜技術(shù)分離化合物，并通過(guò)波譜技術(shù)（包括高分辨質(zhì)譜、一維和二維核磁）結(jié)合文獻(xiàn)對(duì)照確定其結(jié)構(gòu)。Ellman法用于測(cè)試化合物的乙酰膽堿酯酶抑制活性。 結(jié)果：從炭疽菌HK-08中共鑒定了四個(gè)吲哚衍生物，包括6'-hydroxymonaspiloindole (1), 2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl) acetate (2), 2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate (3), 和monaspiloindole (4)。其中化合物4具有弱的乙酰膽堿酯酶抑制活性，其IC50值為69.30 ± 6.27 μmol/L（他克寧為陽(yáng)性對(duì)照，IC50值為69.30 ± 6.27 μmol/L）。 結(jié)論：化合物1?3為新化合物，化合物具有弱的乙酰膽堿酯酶抑制活性。

    Abstract:
    Objective: To study secondary metabolites from endophytic fungus Colletotruchum sp. HK-08 originated from the leaves of Nerium indicum. Methods: The compounds were isolated by various column chromatographic techniques, and their structures were elucidated by spectroscopic techniques [high resolution electrospray ionization mass spectroscopy (HRESIMS), one-dimensional (1D) and two dimensional (2D) nuclear magnetic resonance spectroscopy (NMR)], as well as comparison with literature data. The Ellman method was used to determine the acetylcholinesterase (AChE) inhibitory activity. Results: Four indole derivatives were identified from Colletotruchum sp. HK-08, including 60-hydroxymonaspiloindole (1), 2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl) acetate (2), 2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate (3), and monaspiloindole (4). Compound 4 presented weak AChE inhibitory activity with IC50 value of (69.30 ± 6.27) μmol/L [tacrine as the positive control, with IC50 value of (0.61 ± 0.07) μmol/L]. Conclusion: Compounds 1–3 were new compounds, and compound 4 had weak AChE inhibitory activity.

    關(guān)鍵詞:
    炭疽菌;植物內(nèi)生真菌;吲哚衍生物;夾竹桃;次生代謝產(chǎn)物

    Keywords:


    Project Supported:
    This study was supported by the Hainan Natural Science Foundation (No. 220RC702).

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