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Archive > Volume 16 Issue 2 > 2024,16(2):227-230. DOI:https://doi.org/10.1016/j.chmed.2022.12.009 Prev Next

矮紫杉內(nèi)生菌HDS - Z - 1E的次級(jí)代謝產(chǎn)物及其對(duì)過氧化氫酶的活化作用

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Metabolites isolated from Penicillium HDS-Z-1E, an endophytic fungal strain isolated from Taxus cuspidata and their activation effect of catalase

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    摘要:
    目的：研究從矮紫杉內(nèi)生菌HDS - Z - 1E中分離的化合物及其對(duì)過氧化氫酶的活化作用。 方法：采用硅膠、Sephadex LH-20和HPLC等色譜方法進(jìn)行分離純化，根據(jù)1H-NMR, 13C-NMR, HMBC 和 HMQC等光譜數(shù)據(jù)對(duì)5種代謝物進(jìn)行了結(jié)構(gòu)鑒定。通過分子對(duì)接技術(shù)研究了這5種代謝產(chǎn)物與過氧化氫酶結(jié)合的活化位點(diǎn)。 結(jié)果：從矮紫杉HDS-Z- 1E中分離出5種代謝物（1-5），分別鑒定為4-羥基-4-甲基二氫吡喃酮(4-hydroxy-4-methyltetrahydro-2H-pyran-2-one，1)、4-羥甲基-5，6-二氫吡喃酮(4-hydroxymethyl-5, 6-dihydro-pyran-2-one，2)、4-羧基-5，6-二氫吡喃酮(5, 6-dihydro-2-oxo-2H-pyran-4-carboxylic，3)、N-乙酰肼苯甲酸(N-acetyl-hydrazinobenzoic acid，4)、2-(2，5-二羥苯基)乙酸甲酯(methyl 2-(2, 5-dihydroxyphenyl) acetate，5)。 結(jié)論：化合物3是一種未見報(bào)道的新化合物。化合物3和4可能為過氧化氫酶的潛在激活劑，本研究為過氧化氫酶激活劑的開發(fā)提供了理論基礎(chǔ)。

    Abstract:
    Objective: To study the compounds isolated from Penicillium HDS-Z-1E, an endophytic fungal strain isolated from Taxus cuspidata and their activation effect of catalase (CAT). Methods: The chemical constituents were isolated from Penicillium HDS-Z-1E, by using silica gel, Sephadex LH-20 and HPLC. The structural elucidations of five metabolites were elucidated on the basis of spectroscopic including 1H NMR, 13C NMR, HMBC and HSQC. Their activation sites of catalase have been investigated by molecular docking. Results: Five metabolites, compounds (1–5) were isolated from Penicillium HDS-Z-1E and identified as 4-hydroxy-4-methyltetrahydro-2H-pyran-2-one (1), 4-hydroxymethyl-5, 6-dihydro-pyran-2-one (2), 5, 6-dihydro-2-oxo-2H-pyran-4-carboxylic (3), N-acetyl hydrazinobenzoic acid (4), and methyl 2-(2, 5-dihydroxyphenyl) acetate (5). Conclusion: Compound 3 is a new compound. Compounds 3 and 4 may have potential activators of catalase, providing a theoretical basis for the development of CAT activators.

    關(guān)鍵詞:
    過氧化氫酶激動(dòng)劑;分子模擬;青霉屬;矮紫杉;、4-羧基-5，6-二氫吡喃酮

    Keywords:


    Project Supported:
    This work was supported by Excellent Youth Team for Scientific Research, Innovation and Entre-preneurship of Dalian University (No. XQN202004).

    Clc Number:

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