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Archive > Volume 16 Issue 1 > 2024,16(1):162-167. DOI:https://doi.org/10.1016/j.chmed.2022.11.008 Prev Next

人工栽培蟲草中的化學組分及其抗纖維化作用

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Chemical components in cultivated Cordyceps sinensis and their effects on fibrosis

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    摘要:
    目的：人工栽培蟲草發(fā)酵菌粉是一種具有補肺益腎效果的臨床藥品和保健品，其存在彌補了野生蟲草的嚴重短缺問題。本研究旨在探索人工栽培蟲草中的化學組分并比較其抗纖維化作用。 方法：從人工栽培蟲草中分離純化核苷、甾醇和多糖類組分，分別采用高效液相色譜法、氣相色譜-質(zhì)譜聯(lián)用法和化學顯色法進行分析。以高糖誘導的大鼠腎系膜細胞作為模型，以纖維連接蛋白和1型膠原作為評價指標，比較評估人工栽培冬蟲夏草不同組分的抗纖維化作用。 結(jié)果：人工栽培蟲草中含有10種核苷和1種甾醇，核苷、甾醇和多糖的含量約為2%、0.55%和4.4%。核苷、甾醇和多糖組分表現(xiàn)出不同程度的抗纖維化活性，其中核苷組分和甾醇組分抑制細胞外基質(zhì)表達的效果更好。 結(jié)論：人工栽培蟲草與野生蟲草具有相似的成分，核苷和甾醇可能是其發(fā)揮抗纖維化作用的主要活性物質(zhì)。本研究將為基于人工栽培蟲草開發(fā)高效低毒的藥物提供有價值的信息。

    Abstract:
    Objective: Cultivated Cordyceps sinensis powder has been used as clinical drug and healthy food to nourish the lung and kidney, which solves the problem of serious shortage of wild C. sinensis. This study aims to explore the chemical components and compared their anti-fibrotic effects in cultivated C. sinensis. Methods: Nucleosides, sterols and polysaccharides were separated and purified from cultivated C. sinensis, and analyzed by high performance liquid chromatography, gas chromatography-mass spectrometry and chemical chromogenic methods, respectively. In high glucose-induced rat mesangial cell models, fibronectin and type 1 collagen were used as evaluation indicators. Results: There were 10 kinds of nucleosides and one sterol in cultivated C. sinensis. The contents of nucleosides, sterols and polysaccharides in the cultivated C. sinensis were close to 2%, 0.55% and 4.4%, respectively. Furthermore, nucleoside, sterol and polysaccharide components exhibited varying degrees of antifibrotic activity. The nucleoside components and sterol components inhibited the expression of extracellular matrix more effectively in the three main components. Conclusion: Cultivated C. sinensis remains the similar compounds with the wild C. sinensis, and nucleosides and sterols may be the main active substances that contribute to its anti fibrotic effects. The project of this study may provide valuable information on further optimization of more effective remedies with few side effects based on cultivated C. sinensis.

    關(guān)鍵詞:
    抗纖維化;人工栽培冬蟲夏草;麥角甾醇;核苷;多糖

    Keywords:


    Project Supported:
    This study was supported by Shandong Provincial Natural Science Foundation (No. ZR2021QH296) and the Taishan Scholars Program for Young Expert of Shandong Province (No. tsqn202306383).

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