Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches

doi:https://doi.org/10.1016/j.chmed.2023.07.001

Archive > Volume 16 Issue 1 > 2024,16(1):27-41. DOI:https://doi.org/10.1016/j.chmed.2023.07.001 Prev Next

多維色譜結(jié)合化學(xué)計(jì)量學(xué)方法用于傳統(tǒng)中藥的更深層次研究

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Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches

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摘要:

隨著人們對(duì)化學(xué)合成藥局限性的認(rèn)識(shí)和了解，全球日益重視天然藥物的研究。中藥（CHMs）作為天然藥物的重要分支，具有歷史悠久、理論獨(dú)特、療效顯著和資源豐富等特點(diǎn)。然而，CHMs成分復(fù)雜，藥理機(jī)制及物質(zhì)基礎(chǔ)不明，從而限制了其走向國(guó)際化和現(xiàn)代化。為了更好地理解復(fù)雜CHMs成分并控制其質(zhì)量，強(qiáng)大的分析技術(shù)及數(shù)據(jù)處理工具至關(guān)重要。傳統(tǒng)的一維(1D)色譜方法雖已被廣泛應(yīng)用于CHMs中多組分的分析，但CHMs樣品的復(fù)雜性超過(guò)了任何單一分離模式的最大容量。因此，近幾十年里，許多研究者都在探索獨(dú)立分離技術(shù)的最佳耦合方式，以提高對(duì)復(fù)雜CHMs樣品的分離能力。二維(2D)分離系統(tǒng)應(yīng)運(yùn)而生，其分離柱基于兩個(gè)獨(dú)立的分離機(jī)制，具有比一維分離系統(tǒng)更強(qiáng)大的分離能力，已成功地用于分離和分析CHMs樣品，具有優(yōu)異的性能。且隨著科學(xué)技術(shù)的發(fā)展，更多的多維色譜技術(shù)相繼被提出，并被用于復(fù)雜樣品分析。當(dāng)然，分離能力的提高帶來(lái)了高階數(shù)據(jù)集，也對(duì)化學(xué)計(jì)量數(shù)據(jù)處理工具提出了更高要求。因此，本文旨在綜述多維色譜技術(shù)（主要包括2D-GC和2D-LC）結(jié)合化學(xué)計(jì)量學(xué)在CHMs分析和質(zhì)量評(píng)價(jià)領(lǐng)域中的應(yīng)用，有助于相關(guān)領(lǐng)域人員開展相應(yīng)研究。

Abstract:

Traditional Chinese medicines (TCMs) possess a rich historical background, unique theoretical framework, remarkable therapeutic efficacy, and abundant resources. However, the modernization and internationalization of TCMs have faced significant obstacles due to their diverse ingredients and unknown mechanisms. To gain deeper insights into the phytochemicals and ensure the quality control of TCMs, there is an urgent need to enhance analytical techniques. Currently, two-dimensional (2D) chromatography, which incorporates two independent separation mechanisms, demonstrates superior separation capabilities compared to the traditional one-dimensional (1D) separation system when analyzing TCMs samples. Over the past decade, new techniques have been continuously developed to gain actionable insights from complex samples. This review presents the recent advancements in the application of multidimensional chromatography for the quality evaluation of TCMs, encompassing 2D-gas chromatography (GC), 2D-liquid chromatography (LC), as well as emerging three-dimensional (3D)-GC, 3D-LC, and their associated data-processing approaches. These studies highlight the promising potential of multidimensional chromatographic separation for future phytochemical analysis. Nevertheless, the increased separation capability has resulted in higher-order data sets and greater demands for data-processing tools. Considering that multidimensional chromatography is still a relatively nascent research field, further hardware enhancements and the implementation of chemometric methods are necessary to foster its robust development.

關(guān)鍵詞:

化學(xué)計(jì)量學(xué); 多維分離;離線二維液相色譜法;在線二維液相色譜法;質(zhì)量控制;中藥;在線二維液相色譜法

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We appreciate Zhijun Zhao (J&X Technologies) and Sifan Luo (Guangdong MS Institute of Scientific Instrument Innovation) for their scientific assistance in 2D-GC and ToF MS explanation. This work is financially supported by the Hunan 2011 Collaborative Innovation Center of Chemical Engineering & Technology with Environmental Benignity and Effective Resource Utilization. Additional funding was provided by the Hunan Province Natural Science Fund (No. 2020JJ4569 and 2023JJ60378), and the Hunan Province College Students’ Innovation and Entrepreneurship Training Program (No. S202110530044 and S202210530048). The study has received approval from the university’s review board.

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