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Archive > Volume 15 Issue 1 > 223,15(1):123-131. DOI:https://doi.org/10.1016/j.chmed.2022.04.003 Prev Next

基于譜效關(guān)系識別四君子湯中人參的抗炎成分

Original articles

Identification of anti-inflammatory components in Panax ginseng of Sijunzi Decoction based on spectrum-effect relationship

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    摘要:
    目的:擬在人參臨床應(yīng)用的配伍環(huán)境中識別其主要藥效成分。為此，本研究以廣泛應(yīng)用的中藥復(fù)方四君子湯為載體對人參的抗炎成分進(jìn)行了研究。 方法:采用UPLC技術(shù)建立了以不同來源人參藥材為原料的10批四君子湯的指紋圖譜，研究其化學(xué)成分。同時，通過葡聚糖硫酸鈉誘導(dǎo)的小鼠潰瘍性結(jié)腸炎模型評價其抗炎作用。應(yīng)用灰色關(guān)聯(lián)分析探討四君子湯指紋圖譜與抗炎作用的相關(guān)性。建立脂多糖誘導(dǎo)的RAW264.7小鼠巨噬細(xì)胞炎癥模型，以評價篩選出的人參有效物質(zhì)的抗炎作用。 結(jié)果:灰色關(guān)聯(lián)分析表明，人參中的三七皂苷R1、人參皂苷Rg2、人參皂苷Rb3是四君子湯的主要抗炎活性成分。脂多糖誘導(dǎo)的RAW264.7小鼠巨噬細(xì)胞實驗證明，這三種成分與四君子湯的抗炎過程密切相關(guān)，并顯示出與四君子湯方劑接近的藥效作用。 結(jié)論:本研究為探索中藥復(fù)方中人參的藥理活性成分提供了總體思路，有助于依據(jù)臨床療效建立中藥復(fù)方中中藥材的質(zhì)量標(biāo)準(zhǔn)。

    Abstract:
    Objective: This study aimed to identify the main medicinal active components of Panax ginseng (P. ginseng) in the compatibility environment of clinical application. For this purpose, the anti-inflammatory ingredients of P. ginseng were investigated based on its therapeutic effect in Sijunzi Decoction (SJD) which is a widely used traditional Chinese formula. Methods: The fingerprints of 10 batches of SJD consisting of different sources of P. ginseng were established by UPLC technique to investigate the chemical components. At the same time, the anti-inflammatory effects of these components were evaluated by dextran sulfate sodium-induced ulcerative colitis mouse model. Grey relational analysis was applied to explore the correlation degree between fingerprints and anti-inflammatory effects in SJD. Lipopolysaccharide-stimulated RAW264.7 murine macrophages were established to evaluate the anti-inflammatory action of the screened effective substances of P. ginseng. Results: According to grey relational analysis, notoginsenoside R1, ginsenoside Rg2 and ginsenoside Rb3 of P. ginseng were the major anti-inflammatory contributions in SJD. They had been proven to be closely associated with the anti-inflammatory process of SJD and displayed a close effect compared with SJD by LPS-stimulated RAW264.7 murine macrophages. Conclusion: Our work provides a general strategy for exploring the pharmacological ingredients of P. ginseng in traditional Chinese formulas which is beneficial for establishing the quality standards of traditional herbs in traditional Chinese medicine prescription based on their clinical therapeutic effect.

    關(guān)鍵詞:
    抗炎;人參皂苷;人參;四君子湯;譜效關(guān)系;潰瘍性結(jié)腸炎

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    Project Supported:
    This study was financially supported by Science Foundation of Jilin Educational Committee (No. JJKH20200363KJ). Jilin Science & Technology Development Plan (No. 20190304009YY). Jilin Science & Technology Development Plan (No. 20200404090YY).

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