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Archive > Volume 11 Issue 1 > 2019,11(1):20-27. DOI:10.1016/j.chmed.2018.09.005 Prev Next

黃芩不同部位中15種黃酮類成分的含量測定及化學(xué)計(jì)量學(xué)研究

Original articles

Simultaneous determination of 15 flavonoids from different parts of Scutellaria baicalensis and its chemometrics analysis

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    摘要:
    目的：黃芩的地上部分作為黃芩茶飲用已有近千年的歷史，其主要的活性成分為黃酮類化合物。但目前，尚無黃芩茶質(zhì)量評價(jià)的方法，且其含量相對較高的黃酮類化合物異紅花素-7-O-β-D-葡萄糖醛酸苷、紅花素-7-O-β-D-葡萄糖醛酸苷和異高山黃芩素-8-O-β-D-葡萄糖醛酸苷尚未被定量研究。方法：在HPLC-DAD色譜條件下，對77份樣品（包括34份根、15份莖、15份葉及13份花）樣品進(jìn)行了分析。 同時(shí)，建立了黃芩不同部位的色譜指紋圖譜。 并基于黃芩不同部位中15種黃酮類化合物的含量，進(jìn)行了化學(xué)計(jì)量學(xué)分析，包括主成分分析（PCA）、正交偏最小二乘判別（OPLS-DA）分析和聚類熱圖分析。結(jié)果：結(jié)果表明黃芩不同部位中15種黃酮類化合物的含量及分布情況存在顯著差異。雖然莖和葉中化學(xué)成分成分相似，但莖中主要黃酮類化合物的含量均低于葉片； 異紅花素-7-O-β-D-葡萄糖醛酸苷[（106.66±22.68）mg/g]、紅花素-7-O-β-D-葡萄糖醛酸苷 [（19.82±11.17）mg/g ]和異高山黃芩素-8-O-β-D-葡萄糖醛酸苷[（3.10±1.73）mg / g]的含量在葉中最高；芹菜素-7-O-β-D-吡喃葡萄糖苷和白楊素-7-O-β-D-葡萄糖醛苷酸的含量在花中最高；根部中黃芩苷、黃芩素、漢黃芩苷、漢黃芩素、山姜素和千層紙素A的含量高于其他部位。結(jié)論：該方法經(jīng)過充分驗(yàn)證，可有效地表征黃芩不同部位中主要黃酮類化合物的含量及分布情況。為黃芩茶質(zhì)量評價(jià)體系建立了奠定可靠的基礎(chǔ)。

    Abstract:
    Objective The aerial parts of Scutellaria baicalensis were used as Huangqin Tea for thousands of years and mainly contain flavonoids which contribute to its bioactivities. However, there is no appropriate quality evaluation method of Huangqin Tea, and three flavanones of isocarthamidin-7-O-β-D-glucuronide, carthamidin-7-O-β-D-glucuronide, and isoscutellarein-8-O-β-D-glucuronide with high contents in the aerial parts have never been defined quantitatively. Here, an HPLC-DAD method for simultaneous determination of 15 flavonoids and systematically compared their contents and distribution in the roots, stems, leaves, and flowers of S. baicalensis was established. Methods Under the HPLC-DAD chromatographic conditions, 77 batches of samples of S. baicalensis were analyzed. Meanwhile, the chromatographic fingerprint of different parts of S. baicalensis was established. Subsequently, principal component analysis (PCA), orthogonal projections to latent structures discriminant analysis (OPLS-DA), and clustering heat map were performed based on the contents of 15 flavonoids in different parts of S. baicalensis. Results The results showed significant differences in the contents and distributions of 15 flavonoids among the different parts of S. baicalensis. The chemical composition of stems showed some similarities to leaves, and their contents were all lower than leaves. The contents of isocarthamidin-7-O-β-D-glucuronide [(106.66 ± 22.68) mg/g], carthamidin-7-O-β-D-glucuronide [(19.82 ± 11.17) mg/g], and isoscutellarein-8-O-β-D-glucuronide [(3.10 ± 1.73) mg/g] were the highest in leaves. The content of apigenin-7-O-β-D-glucopyranoside and chrysin-7-O-β-D-glucuronide were the highest in flowers. The contents of baicalin, baicalein, wogonoside, wogonin, alpinetin, and oroxylin A were higher in roots than in other parts. Conclusion The method was fully validated and could be effectively used to characterize the contents and distributions of main flavonoids in the different parts of S. baicalensis. It may lay a foundation to establish the quality evaluation system for Huangqin Tea.

    關(guān)鍵詞:
    黃酮; HPLC; 黃芩茶; 黃芩

    Keywords:


    Project Supported:
    This work would not be accomplished without the support from the Capital Citizens’ Health Project Cultivation (Z161100000116101).

    Clc Number:

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