Effect of n-butanol Extract from Potentilla anserina on Hypoxia- induced Calcium Overload and SERCA2 Expression of Rat Cardiomyocytes

LI Ling-zhi; WANG Lu-jun; WANG Yue; CUI Ying; LI Jian-yu; ZHANG Li; ZHANG Yong-liang; LI Ling-zhi; WANG Lu-jun; WANG Yue; CUI Ying; LI Jian-yu; ZHANG Li; ZHANG Yong-liang

Archive > Volume 4 Issue 2 > 2012,4(2):142-149. DOI:10.3969/j.issn.1674-6384.2012.02.008 Prev Next

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Effect of n-butanol Extract from Potentilla anserina on Hypoxia- induced Calcium Overload and SERCA2 Expression of Rat Cardiomyocytes

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摘要:

Abstract:

Objective To investigate the effect of n-butanol extract from Potentilla anserina (NP) intervention on hypoxia-induced Ca2+ overload and SERCA2 expression of rat cardiomyocytes. Methods Primary cultured myocardial cell from SD neonatal rat (1－3 d) was used in the establishment of hypoxia model. After hypoxia for 3 h, the Ca2+ concentration of myocardial cells was measured with fura-2/AM fluorescent probe, and the biochemical indicator intracellular Ca2+-ATPase was examined and the mRNA and its protective protein levels of the sarcoplasmic reticulum (SR) Ca2+-ATPases (SERCA2) were assayed with RT-PCR, Western-blotting, and immune-cytochemical staining in each group. Results The results showed that NP decreased Ca2+ concentration, increased the activity of Ca2+-ATPase, and improved the mRNA and protein expression of SERCA2 in hypoxia-injured myocardial cells as compared with the model group. Conclusion These results indicate that NP could attenuate the Ca2+ overload. The mechanism might be explained as that NP could elevate the SERCA2 level, increase the activity of myocardium in rats, and further enhance the capacity of SR Ca2+ re-uptake.

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National Natural Science Foundation of China (81073152) and the Great Program of Science Foundation of Tianjin (10JCZDJC21100)

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